Diagnostic PCR: Comparative sensitivity of four probe chemistries

Mathilde Hartmann Josefsen, Charlotta Löfström, Helle Mølgaard Sommer, Jeffrey Hoorfar

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.
Original languageEnglish
JournalMolecular and Cellular Probes
Volume23
Issue number3-4
Pages (from-to)201-203
ISSN0890-8508
DOIs
Publication statusPublished - 2009

Keywords

  • Real-time PCR
  • TaqMan
  • Scorpion
  • MGB
  • Probe
  • LNA

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