Diagnostic PCR: Comparative sensitivity of four probe chemistries

Mathilde Hartmann Josefsen; Charlotta Löfström; Helle Mølgaard Sommer; Jeffrey Hoorfar

doi:10.1016/j.mcp.2009.02.003

Diagnostic PCR: Comparative sensitivity of four probe chemistries

Mathilde Hartmann Josefsen, Charlotta Löfström, Helle Mølgaard Sommer, Jeffrey Hoorfar

National Food Institute

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.

Original language	English
Journal	Molecular and Cellular Probes
Volume	23
Issue number	3-4
Pages (from-to)	201-203
ISSN	0890-8508
DOIs	https://doi.org/10.1016/j.mcp.2009.02.003
Publication status	Published - 2009

Keywords

Real-time PCR
TaqMan
Scorpion
MGB
Probe
LNA

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10.1016/j.mcp.2009.02.003

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title = "Diagnostic PCR: Comparative sensitivity of four probe chemistries",

abstract = "Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.",

keywords = "Real-time PCR, TaqMan, Scorpion, MGB, Probe, LNA",

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AU - Josefsen, Mathilde Hartmann

AU - Löfström, Charlotta

AU - Sommer, Helle Mølgaard

AU - Hoorfar, Jeffrey

PY - 2009

Y1 - 2009

N2 - Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.

AB - Three probe chemistries: locked nucleic acid (LNA), minor groove binder (MGB) and Scorpion were compared with a TaqMan probe in a validated real-time PCR assay for detection of food-borne thermotolerant Campylobacter. The LNA probe produced significantly lower Ct-values and a higher proportion of positive PCR responses analyzing less than 150 DNA copies than the TaqMan probe. Choice of probe chemistry clearly has an impact on the sensitivity of PCR assays, and should be considered in an optimization strategy.

KW - Real-time PCR

KW - TaqMan

KW - Scorpion

KW - MGB

KW - Probe

KW - LNA

U2 - 10.1016/j.mcp.2009.02.003

DO - 10.1016/j.mcp.2009.02.003

M3 - Journal article

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SP - 201

EP - 203

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

IS - 3-4

ER -

Diagnostic PCR: Comparative sensitivity of four probe chemistries

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Keywords

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