Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study

Hina Singh, Juan Du, Priyanka Singh, Gafurjon Tom Mavlonov, Tae Hoo Yi*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5%. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.
Original languageEnglish
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume185
Pages (from-to)100-110
ISSN1011-1344
DOIs
Publication statusPublished - 2018

Keywords

  • Superparamagnetic iron oxide nanoparticles
  • Direct conjugation
  • Ginsenoside CK
  • Ginsenoside Rg3

Cite this

@article{4ff5fb8f7ba7438fa53ee91c489113a7,
title = "Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study",
abstract = "The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5{\%}. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.",
keywords = "Superparamagnetic iron oxide nanoparticles, Direct conjugation, Ginsenoside CK, Ginsenoside Rg3",
author = "Hina Singh and Juan Du and Priyanka Singh and Mavlonov, {Gafurjon Tom} and Yi, {Tae Hoo}",
year = "2018",
doi = "10.1016/j.jphotobiol.2018.05.030",
language = "English",
volume = "185",
pages = "100--110",
journal = "Journal of Photochemistry and Photobiology, B: Biology",
issn = "1011-1344",
publisher = "Elsevier",

}

Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study. / Singh, Hina; Du, Juan; Singh, Priyanka; Mavlonov, Gafurjon Tom; Yi, Tae Hoo.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 185, 2018, p. 100-110.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Development of superparamagnetic iron oxide nanoparticles via direct conjugation with ginsenosides and its in-vitro study

AU - Singh, Hina

AU - Du, Juan

AU - Singh, Priyanka

AU - Mavlonov, Gafurjon Tom

AU - Yi, Tae Hoo

PY - 2018

Y1 - 2018

N2 - The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5%. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.

AB - The current study focused on direct conjugation of superparamagnetic iron oxide nanoparticles (SPIONs) with ginsenosides CK and Rg3. The direct conjugation approach was low-cost, eco-friendly, simple, fast and high yield. The synthesized conjugates (SPION-CK and SPION-Rg3) were characterized by field emission transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, and magnetometer. The characterization results confirmed the formation of SPIONs conjugates. The maximum attaching percentage for ginsenosides to SPIONs was found to be 5%. In vitro cytotoxicity assay in HaCaT keratinocyte cells revealed that the conjugates were non-cytotoxic to normal cells. Moreover, the anti-inflammatory activity of SPION-CK and SPION-Rg3 were investigated. The expression of reactive oxygen species (ROS) in lipopolysaccharide-activated RAW 264.7 (murine macrophage cells) were inhibited by SPIONs conjugates in a dose-dependent manner. In addition, SPION-CK and SPION-Rg3 significantly reduced the production of nitric oxide and inducible nitric oxide synthase (iNOS) in a dose-dependent manner in the lipopolysaccharide-induced RAW 264.7 cells. Overall the results suggested that the SPIONs were conjugated with ginsenosides CK and Rg3 by using direct conjugation approach were non-cytotoxic and can be used as a carrier for intracellular release of ginsenosides in inflammatory diseases.

KW - Superparamagnetic iron oxide nanoparticles

KW - Direct conjugation

KW - Ginsenoside CK

KW - Ginsenoside Rg3

U2 - 10.1016/j.jphotobiol.2018.05.030

DO - 10.1016/j.jphotobiol.2018.05.030

M3 - Journal article

VL - 185

SP - 100

EP - 110

JO - Journal of Photochemistry and Photobiology, B: Biology

JF - Journal of Photochemistry and Photobiology, B: Biology

SN - 1011-1344

ER -