Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae

Ryan M. Phelan, Daniel Sachs, Shayne J. Petkiewicz, Jesus F. Barajas, Jacquelyn M. Blake-Hedges, Mitchell G. Thompson, Amanda Reider Apel, Blake J. Rasor, Leonard Katz, Jay D. Keasling

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Streptomyces have a rich history as producers of important natural products and this genus of bacteria has recently garnered attention for its potential applications in the broader context of synthetic biology. However, the dearth of genetic tools available to control and monitor protein production precludes rapid and predictable metabolic engineering that is possible in hosts such as Escherichia coli or Saccharomyces cerevisiae. In an effort to improve genetic tools for Streptomyces venezuelae, we developed a suite of standardized, orthogonal integration vectors and an improved method to monitor protein production in this host. These tools were applied to characterize heterologous promoters and various attB chromosomal integration sites. A final study leveraged the characterized toolset to demonstrate its use in producing the biofuel precursor bisabolene using a chromosomally integrated expression system. These tools advance S. venezuelae to be a practical host for future metabolic engineering efforts.
Original languageEnglish
JournalA C S Synthetic Biology
Volume6
Issue number1
Pages (from-to)159-166
ISSN2161-5063
DOIs
Publication statusPublished - 2017

Keywords

  • Streptomyces
  • Bisabolene
  • Fluorescent protein
  • Promoter

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