TY - JOUR
T1 - Development of Lion's Mane Mushroom Extract-Loaded Polyvinyl Alcohol/Chitosan Hydrogel Film Composites for Controlled Release of Ergosterol
AU - Joradon, Pinida
AU - Rungsardthong, Vilai
AU - Thumthanaruk, Benjawan
AU - Vatanyoopaisarn, Savitri
AU - Ruktanonchai, Uracha
AU - Suttisintong, Khomson
AU - Mendes, Ana C.
PY - 2025
Y1 - 2025
N2 - Ergosterol is the most prevalent sterol in microbial membranes, which shows a hypoglycemic effect that can be used to treat type 2 diabetes. However, ergosterol has restricted bioavailability and low free-form solubility in hydrophilic conditions. This research aimed to evaluate the properties of bioactive compounds, ergosterol, from Lion's Mane mushroom (LM) mushroom extract and entrap them in polyvinyl alcohol (PVA) and chitosan (CS) hydrogel film composites with/without the crosslinker tetraethoxysilane (TEOS) to increase the solubility of ergosterol. Ergosterol-enriched extract (37.17 mg/g extract) was extracted from the LM by supercritical fluid extraction using CO2 (SCFE-CO2). LM extract showed no cytotoxic effects on the fibroblast cells, with cell viability ranging from 94.60-97.40%, increased cell proliferation, and wound-healing activity at 1 mg/mL of LM extract. Scanning Electron Microscopy revealed that the film exhibited a homogenous structure with swelling ability. The release rate of ergosterol from the film was relatively higher during 30 to 60 min of submersion in phosphate buffer. LM extract-loaded PVA/CS hydrogel film composites have a high potential for wound-healing applications in medical settings. This research challenges further investigation for alternative treatments and offering aids for efficiency improvement in wound-healing. The longer release rate of LM extracts should be investigated in future work.
AB - Ergosterol is the most prevalent sterol in microbial membranes, which shows a hypoglycemic effect that can be used to treat type 2 diabetes. However, ergosterol has restricted bioavailability and low free-form solubility in hydrophilic conditions. This research aimed to evaluate the properties of bioactive compounds, ergosterol, from Lion's Mane mushroom (LM) mushroom extract and entrap them in polyvinyl alcohol (PVA) and chitosan (CS) hydrogel film composites with/without the crosslinker tetraethoxysilane (TEOS) to increase the solubility of ergosterol. Ergosterol-enriched extract (37.17 mg/g extract) was extracted from the LM by supercritical fluid extraction using CO2 (SCFE-CO2). LM extract showed no cytotoxic effects on the fibroblast cells, with cell viability ranging from 94.60-97.40%, increased cell proliferation, and wound-healing activity at 1 mg/mL of LM extract. Scanning Electron Microscopy revealed that the film exhibited a homogenous structure with swelling ability. The release rate of ergosterol from the film was relatively higher during 30 to 60 min of submersion in phosphate buffer. LM extract-loaded PVA/CS hydrogel film composites have a high potential for wound-healing applications in medical settings. This research challenges further investigation for alternative treatments and offering aids for efficiency improvement in wound-healing. The longer release rate of LM extracts should be investigated in future work.
KW - Chitosan
KW - Ergosterol
KW - Hydrogel
KW - Polyvinyl alcohol
KW - Tetraethoxysilane
U2 - 10.14416/j.asep.2024.08.008
DO - 10.14416/j.asep.2024.08.008
M3 - Journal article
SN - 2673-0421
VL - 18
JO - Applied Science and Engineering Progress
JF - Applied Science and Engineering Progress
IS - 1
M1 - 7496
ER -