Development of Electrochemical Cantilever Sensors for DNA Applications

Xueling Quan; Arto Heiskanen; Sun Yi; Aleks Labuda; Anders Wolff; Jorge Dulanto; Peter Grutter; Maria Tenje; Anja Boisen

doi:10.1149/05012.0077ecst

Development of Electrochemical Cantilever Sensors for DNA Applications

Xueling Quan, Arto Heiskanen, Sun Yi, Aleks Labuda, Anders Wolff, Jorge Dulanto, Peter Grutter, Maria Tenje, Anja Boisen

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Abstract

In this work, we develop a generic DNA based sensing platform used for characterizing surface functionalization and detecting DNA hybridization. Silicon nitride cantilever sensors are fabricated with an integrated three-electrode system and integrated in a microfluidic chip. Cantilevers with gold electrodes are functionalized with thiol-modified single stranded DNA (ssDNA) probes to detect target DNA. During functionalization and hybridization, information related to nanomechanical changes on the surface are obtained by optical measurements of changes in cantilever deflection. Simultaneously, the process is monitored electrochemically. The results clearly indicate that the electrochemical cantilever sensor is very sensitive for detecting DNA hybridization at the cantilever surface.

Original language	English
Journal	E C S Transactions
Volume	50
Pages (from-to)	2077-81
ISSN	1938-5862
DOIs	https://doi.org/10.1149/05012.0077ecst
Publication status	Published - 2013

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1 Participating in or organising a conference

222th Electrochmical Society meeting
Xueling Quan (Participant)
7 Oct 2012 → 12 Oct 2012
Activity: Attending an event › Participating in or organising a conference

Cite this

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title = "Development of Electrochemical Cantilever Sensors for DNA Applications",

abstract = "In this work, we develop a generic DNA based sensing platform used for characterizing surface functionalization and detecting DNA hybridization. Silicon nitride cantilever sensors are fabricated with an integrated three-electrode system and integrated in a microfluidic chip. Cantilevers with gold electrodes are functionalized with thiol-modified single stranded DNA (ssDNA) probes to detect target DNA. During functionalization and hybridization, information related to nanomechanical changes on the surface are obtained by optical measurements of changes in cantilever deflection. Simultaneously, the process is monitored electrochemically. The results clearly indicate that the electrochemical cantilever sensor is very sensitive for detecting DNA hybridization at the cantilever surface.",

author = "Xueling Quan and Arto Heiskanen and Sun Yi and Aleks Labuda and Anders Wolff and Jorge Dulanto and Peter Grutter and Maria Tenje and Anja Boisen",

year = "2013",

doi = "10.1149/05012.0077ecst",

language = "English",

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pages = "2077--81",

journal = "E C S Transactions",

issn = "1938-5862",

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TY - JOUR

T1 - Development of Electrochemical Cantilever Sensors for DNA Applications

AU - Quan, Xueling

AU - Heiskanen, Arto

AU - Yi, Sun

AU - Labuda, Aleks

AU - Wolff, Anders

AU - Dulanto, Jorge

AU - Grutter, Peter

AU - Tenje, Maria

AU - Boisen, Anja

PY - 2013

Y1 - 2013

N2 - In this work, we develop a generic DNA based sensing platform used for characterizing surface functionalization and detecting DNA hybridization. Silicon nitride cantilever sensors are fabricated with an integrated three-electrode system and integrated in a microfluidic chip. Cantilevers with gold electrodes are functionalized with thiol-modified single stranded DNA (ssDNA) probes to detect target DNA. During functionalization and hybridization, information related to nanomechanical changes on the surface are obtained by optical measurements of changes in cantilever deflection. Simultaneously, the process is monitored electrochemically. The results clearly indicate that the electrochemical cantilever sensor is very sensitive for detecting DNA hybridization at the cantilever surface.

AB - In this work, we develop a generic DNA based sensing platform used for characterizing surface functionalization and detecting DNA hybridization. Silicon nitride cantilever sensors are fabricated with an integrated three-electrode system and integrated in a microfluidic chip. Cantilevers with gold electrodes are functionalized with thiol-modified single stranded DNA (ssDNA) probes to detect target DNA. During functionalization and hybridization, information related to nanomechanical changes on the surface are obtained by optical measurements of changes in cantilever deflection. Simultaneously, the process is monitored electrochemically. The results clearly indicate that the electrochemical cantilever sensor is very sensitive for detecting DNA hybridization at the cantilever surface.

U2 - 10.1149/05012.0077ecst

DO - 10.1149/05012.0077ecst

M3 - Journal article

VL - 50

SP - 2077

EP - 2081

JO - E C S Transactions

JF - E C S Transactions

SN - 1938-5862

ER -

Development of Electrochemical Cantilever Sensors for DNA Applications

Abstract

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222th Electrochmical Society meeting

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