Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution

Tomas Strucko, Katharina Zirngibl, Elsayed Tharwat Tolba Mohamed, Adam Feist, Kiran R. Patil, Jochen Förster

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

Abstract

With increasing interest in biosustainable technologies, the need for converting available non-saccharide carbon sources most efficiently is emerging. Highly abundant crude glycerol, a major waste residue in biodiesel production, has attracted attention as a cheap carbon source for microbial fermentation processes. The most commonly known microbial cell factory, the yeast Saccharomyces cerevisiae, has been extensively applied for the production of a wide range of scientifically and industrially relevant products using saccharides (mainly glucose) as carbon source. However, it was shown that popular wild-type laboratory yeast strains, commonly applied in metabolic engineering studies, did not grow or grew very slowly in glycerol medium.In this work, an adaptive laboratory evolution approach to obtain S. cerevisiae strains with an improved ability to grow on glycerol was applied. A broad array of evolved strains, which exhibited a significant increase in the specific growth rate and a higher glycerol consumption rate, were isolated. The best performing strains were further analyzed by classical genetics and whole genome re-sequencing in order to understand the molecular basis of glycerol catabolism in yeast. The knowledge acquired in this study may be further applied for rational S. cerevisiae strain improvement for using glycerol as a carbon source in industrial biotechnology processes. This work is a part of the DeYeastLibrary consortium financed by ERA-IB DeYeastLibrary - Designer yeast strain library optimized for metabolic engineering applications http://www.era-ib.net/deyeast-library
Original languageEnglish
Title of host publicationThe Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts
Place of PublicationCopenhagen
Publication date2015
Pages22-22
Publication statusPublished - 2015
EventThe Danish Microbiological Society Annual Congress 2015 - Eigtved's Pakhus, Copenhagen, Denmark
Duration: 9 Nov 20159 Nov 2015

Conference

ConferenceThe Danish Microbiological Society Annual Congress 2015
LocationEigtved's Pakhus
CountryDenmark
CityCopenhagen
Period09/11/201509/11/2015

Cite this

Strucko, T., Zirngibl, K., Tharwat Tolba Mohamed, E., Feist, A., Patil, K. R., & Förster, J. (2015). Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts (pp. 22-22). Copenhagen.
Strucko, Tomas ; Zirngibl, Katharina ; Tharwat Tolba Mohamed, Elsayed ; Feist, Adam ; Patil, Kiran R. ; Förster, Jochen. / Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution. The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, 2015. pp. 22-22
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Strucko, T, Zirngibl, K, Tharwat Tolba Mohamed, E, Feist, A, Patil, KR & Förster, J 2015, Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution. in The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, pp. 22-22, The Danish Microbiological Society Annual Congress 2015, Copenhagen, Denmark, 09/11/2015.

Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution. / Strucko, Tomas; Zirngibl, Katharina; Tharwat Tolba Mohamed, Elsayed; Feist, Adam; Patil, Kiran R.; Förster, Jochen.

The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen, 2015. p. 22-22.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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AB - With increasing interest in biosustainable technologies, the need for converting available non-saccharide carbon sources most efficiently is emerging. Highly abundant crude glycerol, a major waste residue in biodiesel production, has attracted attention as a cheap carbon source for microbial fermentation processes. The most commonly known microbial cell factory, the yeast Saccharomyces cerevisiae, has been extensively applied for the production of a wide range of scientifically and industrially relevant products using saccharides (mainly glucose) as carbon source. However, it was shown that popular wild-type laboratory yeast strains, commonly applied in metabolic engineering studies, did not grow or grew very slowly in glycerol medium.In this work, an adaptive laboratory evolution approach to obtain S. cerevisiae strains with an improved ability to grow on glycerol was applied. A broad array of evolved strains, which exhibited a significant increase in the specific growth rate and a higher glycerol consumption rate, were isolated. The best performing strains were further analyzed by classical genetics and whole genome re-sequencing in order to understand the molecular basis of glycerol catabolism in yeast. The knowledge acquired in this study may be further applied for rational S. cerevisiae strain improvement for using glycerol as a carbon source in industrial biotechnology processes. This work is a part of the DeYeastLibrary consortium financed by ERA-IB DeYeastLibrary - Designer yeast strain library optimized for metabolic engineering applications http://www.era-ib.net/deyeast-library

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Strucko T, Zirngibl K, Tharwat Tolba Mohamed E, Feist A, Patil KR, Förster J. Development Of An Efficient Glycerol Utilizing Saccharomyces Cerevisiae Strain Via Adaptive Laboratory Evolution. In The Danish Microbiological Society Annual Congress 2015: Programme & Abstracts. Copenhagen. 2015. p. 22-22