Development of an automated flow-based spectrophotometric immunoassay for continuous detection of zearalenone

Jongjit Jantra*, Kinga Zor, Melanie Sanders, Sarah De Saeger, Martin Hedström, Bo Mattiasson

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Considering the widespread contaminations of food products with mycotoxins, it is important to develop, robust, time- and cost-effective detection methods. We developed and optimized an immunoassay using a continuous flow system for the detection of zearalenone (ZEN). The assay was performed in a flow mode using an automated sequential injection system. Time for an assay cycle was 18 Min. Under optimal conditions, the limit for quantification for ZEN was 0.40 µg L-1 with a linear dependency between concentration and signal amplitude between 0.10 and 10 µg L-1 . The assay proved to be robust and reliable with 13% relative standard deviation between measurements. By dissociating the antigen-antibody complex using a regeneration solution, we showed 50 times reusability of the immobilized antibodies without affecting the antigen-binding properties.
Original languageEnglish
JournalBiotechnology and Applied Biochemistry
Number of pages9
ISSN0885-4513
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Bioanalysis
  • Flow-ELISA
  • Immunoassay
  • Mycotoxin
  • Sequential injection
  • Zearalenone

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