Development of a VHH-Based Erythropoietin Quantification Assay

Stefan Kol, Thomas Beuchert Kallehauge, Simon Adema, Pim Hermans

Research output: Contribution to journalJournal articleResearchpeer-review

349 Downloads (Pure)

Abstract

Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting.
Original languageEnglish
JournalMolecular Biotechnology
Volume57
Issue number8
Pages (from-to)692-700
Number of pages9
ISSN1073-6085
DOIs
Publication statusPublished - 2015

Keywords

  • Biolayer interferometry
  • Chinese hamster ovary (CHO) cells
  • EPO
  • Erythropoietin
  • Quantification assay
  • VHH

Fingerprint

Dive into the research topics of 'Development of a VHH-Based Erythropoietin Quantification Assay'. Together they form a unique fingerprint.

Cite this