Development of a VHH-Based Erythropoietin Quantification Assay

Stefan Kol, Thomas Beuchert Kallehauge, Simon Adema, Pim Hermans

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Erythropoietin (EPO) quantification during cell line selection and bioreactor cultivation has traditionally been performed with ELISA or HPLC. As these techniques suffer from several drawbacks, we developed a novel EPO quantification assay. A camelid single-domain antibody fragment directed against human EPO was evaluated as a capturing antibody in a label-free biolayer interferometry-based quantification assay. Human recombinant EPO can be specifically detected in Chinese hamster ovary cell supernatants in a sensitive and pH-dependent manner. This method enables rapid and robust quantification of EPO in a high-throughput setting.
Original languageEnglish
JournalMolecular Biotechnology
Issue number8
Pages (from-to)692-700
Number of pages9
Publication statusPublished - 2015


  • Biolayer interferometry
  • Chinese hamster ovary (CHO) cells
  • EPO
  • Erythropoietin
  • Quantification assay
  • VHH


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