Development of a new chromogenic method for putrescine quantification using coupling reactions involving putrescine oxidase

Yuta Sugiyama*, Hirokazu Ohta, Rika Hirano, Hiromi Shimokawa, Mikiyasu Sakanaka, Takashi Koyanagi, Shin Kurihara

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Quantification of polyamines, including putrescine, is generally performed using high-performance liquid chromatography (HPLC) or gas chromatography. However, these methods are time-consuming because of sample derivatization and analytical reagent preparation. In this study, we developed a simple and high-throughput putrescine quantification method on a 96-well microtiter plate using putrescine oxidase from Rhodococcus erythropolis NCIMB 11540, peroxidase, 4-aminoantipyrine, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt. The developed method (named as PuO-POD-4AA-TOPS method) was applicable to bacterial culture supernatants. Furthermore, putrescine concentrations determined by the developed method roughly corresponded to the concentrations determined by HPLC.
Original languageEnglish
Article number113607
JournalAnalytical Biochemistry
Volume593
Number of pages4
ISSN0003-2697
DOIs
Publication statusPublished - 2020

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