Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

T. Ito, Niels Jørgen Olesen, Helle Frank Skall, M. Sano, J. Kurita, K. Nakajima, T. Iida

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.
    Original languageEnglish
    JournalDiseases of Aquatic Organisms
    Volume89
    Issue number1
    Pages (from-to)17-27
    ISSN0177-5103
    DOIs
    Publication statusPublished - 2010

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