Development of a fiber-based microfluidic flow cytometry platform using viscoelastic fluids for polydisperse particle suspensions

Murat Serhatlioglu, Emil Alstrup Jensen, Maria Niora, Anne Todsen Hansen, Christian Friberg Nielsen, Michelle Maria Theresia Jansman, Leticia Hosta-Rigau, Morten Hanefeld Dziegiel, Kirstine Berg-Sørensen, Ian D. Hickson, Anders Kristensen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review


Flow cytometry (FC) is a pivotal tool for studying the physical and chemical properties of particles. State-of-the-art FC systems are highly advanced, yet they are expensive, bulky, and require high sample volume, qualified operators, and periodic maintenance. The manipulation of particles suspended in viscoelastic fluids has received increasing attention, especially for miniaturized flow cytometry technologies. This study presents a miniaturized optical capillary FC device using the viscoelastic focusing technique. A straight, one inlet/outlet microcapillary device is precisely aligned to a fiber-coupled laser source and detectors. Forward scattered, side scattered, and fluorescently emitted light signals are collected and analyzed in a real-time environment. The developed platform fits onto an inverted microscope stage enabling real-time microscopy imaging of the particles of interest together with the flow cytometry analysis. We achieved stable viscoelastic focusing and performed FC measurements for rigid polystyrene beads (diameters: 2 - 15 μm), non-spherical human erythrocytes, and canonical shape metaphase human chromosomes. We performed cytometry measurements with a throughput of 100 events/s yielding a coefficient of variation of 2%. This newly developed FC device is a versatile tool and can be operated with any inverted microscope to get the mutual benefits of optical and imaging FC measurements. Furthermore, it is possible to extend these benefits by adding more back-end tools, such as optical trapping and Raman spectroscopy.

Original languageEnglish
Title of host publicationProceedings of Optical Trapping and Optical Micromanipulation XIX
Number of pages9
Publication date2022
Article number1219802
Publication statusPublished - 2022
EventSPIE Nanoscience + Engineering 2022 - San Diego, United States
Duration: 21 Aug 202226 Aug 2022


ConferenceSPIE Nanoscience + Engineering 2022
Country/TerritoryUnited States
CitySan Diego


  • Blood cells
  • Capillary
  • Chromosomes
  • Flow cytometry
  • Microfluidics
  • Optofluidics
  • Viscoelastic focusing


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