Development of a compact Bio-Optofluidic Cell Sorter

Andrew Rafael Bañas, Darwin Palima, Finn Pedersen, Jesper Glückstad

Research output: Contribution to journalConference articleResearchpeer-review


We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.
Original languageEnglish
JournalProceedings of SPIE, the International Society for Optical Engineering
Pages (from-to)82740N
Publication statusPublished - 2012
EventSPIE Photonics West - The Moscone Center, San Francisco, California, United States
Duration: 21 Jan 201226 Jan 2012


ConferenceSPIE Photonics West
LocationThe Moscone Center
Country/TerritoryUnited States
CitySan Francisco, California


  • Optical tweezers or optical manipulation
  • Machine vision
  • Spatial light modulators
  • Image processing
  • Optical sorting


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