TY - JOUR
T1 - Development of a Bacterial Biosensor for Rapid Screening of Yeast p-Coumaric Acid Production
AU - Siedler, Solvej
AU - Khatri, Narendar K.
AU - Zsohar, Andrea
AU - Kjærbølling, Inge
AU - Vogt, Michael
AU - Hammar, Petter
AU - Nielsen, Christian Førgaard
AU - Marienhagen, Jan
AU - Sommer, Morten Otto Alexander
AU - Joensson, Haakan N.
PY - 2017
Y1 - 2017
N2 - Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
AB - Transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. Here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. We describe the effects of different translation initiation rates on the dynamic range of a p-coumaric acid biosensor based on the Bacillus subtilis transcriptional repressor PadR by varying its ribosomal binding site. Furthermore, we demonstrate the functionality of this p-coumaric acid biosensor in Escherichia coli and Corynebacterium glutamicum. Finally, we encapsulate yeast p-coumaric acid-producing cells with E. coli-biosensing cells in picoliter droplets and, in a microfluidic device, rapidly sort droplets containing yeast cells producing high amounts of extracellular p-coumaric acid using the fluorescent E. coli biosensor signal. As additional biosensors become available, such approaches will find broad applications for screening of an extracellular product.
U2 - 10.1021/acssynbio.7b00009
DO - 10.1021/acssynbio.7b00009
M3 - Journal article
C2 - 28532147
SN - 2161-5063
VL - 6
SP - 1860
EP - 1869
JO - A C S Synthetic Biology
JF - A C S Synthetic Biology
IS - 10
ER -