DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

Marianne Jakobsen; Jens Nielsen

doi:10.1016/0378-1135(95)00062-F

DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

Marianne Jakobsen, Jens Nielsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.

Original language	English
Journal	Veterinary Microbiology
Volume	47
Issue number	1-2
Pages (from-to)	191-197
ISSN	0378-1135
DOIs	https://doi.org/10.1016/0378-1135(95)00062-F
Publication status	Published - 1995

Keywords

PIG
ACTINOBACILLUS PLEUROPNEUMONIAE
CULTURE METHODS
INDICATIVE AGAR
SELECTIVE AGAR
TONSIL

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@article{010f53c243ff4b458ca55234c75be9fe,

title = "DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS",

abstract = "In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.",

keywords = "PIG, ACTINOBACILLUS PLEUROPNEUMONIAE, CULTURE METHODS, INDICATIVE AGAR, SELECTIVE AGAR, TONSIL",

author = "Marianne Jakobsen and Jens Nielsen",

year = "1995",

doi = "10.1016/0378-1135(95)00062-F",

language = "English",

volume = "47",

pages = "191--197",

journal = "Veterinary Microbiology",

issn = "0378-1135",

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T1 - DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

AU - Jakobsen, Marianne

AU - Nielsen, Jens

PY - 1995

Y1 - 1995

N2 - In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.

AB - In order to isolate ActinobacillIus pleuropneumoniae from mixed bacterial flora a selective and indicative medium was developed. The optimal concentrations of antibiotics were determined for selective chocolate agar (S-TSA) and selective blood agar (S-MBA) using a set of 25 strains of A. pleuropneumoniae. Following optimization of the media 96 isolates of A. pleuropneumonine were tested on S-TSA and S-MBA. For isolation of A. pleuropneumoniae from 101 pig tonsils collected at slaughter the S-MBA proved significantly better than both S-TSA and the two non-selective agars tested. Furthermore, contaminating growth by bacteria other than A. pleuropneumoniae was significantly reduced on S-MBA compared to S-TSA and the two non-selective media.

KW - PIG

KW - ACTINOBACILLUS PLEUROPNEUMONIAE

KW - CULTURE METHODS

KW - INDICATIVE AGAR

KW - SELECTIVE AGAR

KW - TONSIL

U2 - 10.1016/0378-1135(95)00062-F

DO - 10.1016/0378-1135(95)00062-F

M3 - Journal article

SN - 0378-1135

VL - 47

SP - 191

EP - 197

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 1-2

ER -

DEVELOPMENT AND EVALUATION OF A SELECTIVE AND INDICATIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-PLEUROPNEUMONIAE FROM TONSILS

Abstract

Keywords

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