Determination of reactor operation for the microbial hydroxylation of toluene in a two-liquid phase process

AM Collins, John Woodley, JM Liddell

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Application of biotransformations to the synthesis of industrial chemicals is in part limited by a number of process challenges. We discuss the conversion of toxic, poorly water-soluble organic substrates by whole cells, using as an illustrative example the specific hydroxylation of toluene to toluene cis-glycol by Pseudomonas putida UV4. Toxic effects may be eliminated through the introduction of tetradecane, to partition toluene away from the biocatalyst, to give product concentrations of 30-60 g L(-1), in a two-liquid-phase reactor. The operational limits of this system have been experimentally determined and are presented in the form of windows of operation.
Original languageEnglish
JournalJournal of Industrial Microbiology and Biotechnology
Issue number5
Pages (from-to)382-388
Publication statusPublished - May 1995

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