Application of biotransformations to the synthesis of industrial chemicals is in part limited by a number of process challenges. We discuss the conversion of toxic, poorly water-soluble organic substrates by whole cells, using as an illustrative example the specific hydroxylation of toluene to toluene cis-glycol by Pseudomonas putida UV4. Toxic effects may be eliminated through the introduction of tetradecane, to partition toluene away from the biocatalyst, to give product concentrations of 30-60 g L(-1), in a two-liquid-phase reactor. The operational limits of this system have been experimentally determined and are presented in the form of windows of operation.
|Journal||Journal of Industrial Microbiology and Biotechnology|
|Publication status||Published - May 1995|
Collins, AM., Woodley, J., & Liddell, JM. (1995). Determination of reactor operation for the microbial hydroxylation of toluene in a two-liquid phase process. Journal of Industrial Microbiology and Biotechnology, 14(5), 382-388. https://doi.org/10.1007/BF01569955