Determination of Mass Spectrometric Sensitivity of Different Metalloporphyrin Esters Relative to Porphyrin Ester

Elfinn Larsen, Helge Egsgaard, J. Møller, T. K. With

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Quantitative determination of metalloporphyrin contamination in preparations of biologically important porphyrins was achieved mass spectrometrically by application of the integrated ion current technique. For this purpose, the relative molecular ion sensitivities of the contaminating metal complexes were determined from the ratios of the integrated molecular ion currents of a series of calibration samples containing a porphyrin ester and one of its metal complexes in known molar ratio. Complexes formed with divalent ions of Cu, Zn, Fe, Co and Ni of copro- as well as uro-prophyrin permethylester were all found to have the same molecular ion sensitivities as their metal-free porphyrin ester. The relative metalloporphyrin ester content in a sample of porphyrin ester was thus obtained directly as the integrated ion current ratios of the normalized molecular ions. The preparation of calibration samples, including complex formation and the mass spectrometric methodology, is described and discussed.
    Original languageEnglish
    JournalBIOMEDICAL MASS SPECTROMETRY
    Volume4
    Issue number2
    Pages (from-to)113-117
    ISSN0306-042X
    DOIs
    Publication statusPublished - 1977

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