Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)

Jens Knudsen; Maiken Camilla Trolle Wadum; Jens Kristian Munk; Thomas B. F. Neergaard

Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)

Jens Knudsen (Inventor), Maiken Camilla Trolle Wadum (Inventor), Jens Kristian Munk (Inventor), Thomas B. F. Neergaard (Inventor)

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Abstract

The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.

Original language	English
IPC	C12N15/62; G01N33/573; G01N33/92;
Patent number	WO2001DK00701
Filing date	24/10/2001
Country/Territory	International Bureau of the World Intellectual Property Organization (WIPO)
Priority date	10/11/2000
Priority number	DK20000001683
Publication status	Published - 2002

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title = "Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)",

abstract = "The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.",

author = "Jens Knudsen and Wadum, {Maiken Camilla Trolle} and Munk, {Jens Kristian} and Neergaard, {Thomas B. F.}",

year = "2002",

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type = "Patent",

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TY - PAT

T1 - Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)

AU - Knudsen, Jens

AU - Wadum, Maiken Camilla Trolle

AU - Munk, Jens Kristian

AU - Neergaard, Thomas B. F.

PY - 2002

Y1 - 2002

N2 - The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.

AB - The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.

M3 - Patent

M1 - WO2001DK00701

Y2 - 2001/10/24

ER -

Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)

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