Determination of hydrophobic coenzyme a esters and other lipids using a biosensor comprising a modified coenzyme a- and acyl-coa binding protein (acbp)

Jens Knudsen (Inventor), Maiken Camilla Trolle Wadum (Inventor), Jens Kristian Munk (Inventor), Thomas B. F. Neergaard (Inventor)

    Research output: Patent

    88 Downloads (Pure)

    Abstract

    The invention relates to a biochemical assay for wide class of hydrophobic Coenzyme A esters wherein the analyte is caused to react with a specifically binding, modified protein, and thereby causing a detectable signal. A one step assay for hydrophobic carboxylic acid esters in whole blood, serum, food and feed preparations, tissue extracts, acyl-CoA synthetase reaction media and various laboratory conditions using a modified Coenzyme A- and acyl-CoA binding protein (ACBP) is provided. Furthermore the invention relates to a construct comprising a peptide and a signal moiety for performing an assay, a kit for assaying hydrophobic CoA esters, hydrophobic carboxylic acids, triacylglycerides, phospholipids, and cholesterolesters.
    Original languageEnglish
    IPCC12N15/62; G01N33/573; G01N33/92;
    Patent numberWO2001DK00701
    Filing date24/10/2001
    Country/TerritoryInternational Bureau of the World Intellectual Property Organization (WIPO)
    Priority date10/11/2000
    Priority numberDK20000001683
    Publication statusPublished - 2002

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