Determination of Formaldehyde in Frozen Fish with Formaldehyde Dehydrogenase Using a Flow Injection System with an Incorporated Gel-filtration Chromatography Column

Iben Ellegaard Bechmann

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    Abstract

    A flow injection analysis (FIA) system for determination of formaldehyde in frozen fish products is described. The system provides a rapid and selective determination of formaldehyde in aqueous fish extracts by the combination of a deproteinization procedure and a stopped-flow enzymatic approach in a FIA system. The FIA system is furnished with a gel-filtration chromatography column for on-line removal of the proteins from the extract before the enzymatic analysis is performed. Compared with the standard methods for determination of formaldehyde in fish products the present method is much faster and less affected by interferences. The limit of detection for the proposed method is 2.5 mg/l of formaldehyde. The sampling frequency is about 10 determinations per hour.
    Original languageEnglish
    JournalAnalytica Chimica Acta
    Volume320
    Issue number2-3
    Pages (from-to)155-166
    ISSN0003-2670
    Publication statusPublished - 1996

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