Determination of 5 '-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs

M.B. Oleksiewicz, Anette Bøtner, Jens Nielsen, Torben Storgaard

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We determined the untranslated 5'-leader sequence for three different isolates of porcine reproductive and respiratory syndrome virus (PRRSV): pathogenic European- and American-types, as well as an American-type vaccine strain. 5'-leader from European- and American-type PRRSV differed in length (220 and 190 nt, respectively), and exhibited only approximately 50% nucleotide homology. Nevertheless, highly conserved areas were identified in the leader of all 3 PRRSV isolates, which constitute candidate motifs for binding of protein(s) involved in viral replication. These comparative data provide a priori knowledge for mutational identification of virulence determinants in the 5' nontranslated part of the PRRSV genome.
    Original languageEnglish
    JournalArchives of Virology
    Volume144
    Issue number5
    Pages (from-to)981-987
    ISSN0304-8608
    DOIs
    Publication statusPublished - 1999

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