Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark

Anette Marie Hammerum, Dorthe Sandvag, Sigrid R. Andersen, Anne Mette Seyfarth, Lone Jannok Porsbo, Niels Frimodt-Møller, Ole Eske Heuer

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The occurrence of sulphonamide resistance was investigated in 998 Escherichia coli isolates, obtained from pig faeces collected at slaughter, Danish pork collected at retail outlets and from faeces from healthy persons in Denmark. In total 18% (n = 35), 20% (n = 38) and 26% (n = 161) of the E. coli isolates obtained from humans, pork and pigs, respectively, were resistant to sulphonamide. All sulphonamide resistant E. coli isolates were investigated for the presence of sul1, sul2, sul3 and intl1 genes by PCR. The sul1 gene was detected in 40% (n = 14), 29% (n = 11) and 55% (n = 88) of the sulphonamide resistant isolates from humans, pork and pigs, respectively. The sul2 gene was detected in 80% (n = 28), 76% (n = 29) and 50% (n = 81) of isolates from humans, pork and pigs, respectively. None of the human isolates were PCR-positive for sul3, whereas sul3 was present in 5% of the pork isolates and 11% of the pig isolates. Of the 113 sul1 positive isolates, 97 carried the integron-associated integrase gene hall. All 20 sul3 positive isolates were positive for intl1, and in 12 of these isolates sul3 was the only sulphonamide resistance gene detected. The origin of sul1 and sul2 found in isolates from healthy humans is speculative, but their spread from pigs to humans via the food chain is possible.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Volume106
Issue number2
Pages (from-to)235-237
ISSN0168-1605
DOIs
Publication statusPublished - 2006

Keywords

  • food animals
  • resistance genes
  • antibiotic resistance
  • integrons

Cite this

Hammerum, Anette Marie ; Sandvag, Dorthe ; Andersen, Sigrid R. ; Seyfarth, Anne Mette ; Porsbo, Lone Jannok ; Frimodt-Møller, Niels ; Heuer, Ole Eske. / Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark. In: International Journal of Food Microbiology. 2006 ; Vol. 106, No. 2. pp. 235-237.
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title = "Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark",
abstract = "The occurrence of sulphonamide resistance was investigated in 998 Escherichia coli isolates, obtained from pig faeces collected at slaughter, Danish pork collected at retail outlets and from faeces from healthy persons in Denmark. In total 18{\%} (n = 35), 20{\%} (n = 38) and 26{\%} (n = 161) of the E. coli isolates obtained from humans, pork and pigs, respectively, were resistant to sulphonamide. All sulphonamide resistant E. coli isolates were investigated for the presence of sul1, sul2, sul3 and intl1 genes by PCR. The sul1 gene was detected in 40{\%} (n = 14), 29{\%} (n = 11) and 55{\%} (n = 88) of the sulphonamide resistant isolates from humans, pork and pigs, respectively. The sul2 gene was detected in 80{\%} (n = 28), 76{\%} (n = 29) and 50{\%} (n = 81) of isolates from humans, pork and pigs, respectively. None of the human isolates were PCR-positive for sul3, whereas sul3 was present in 5{\%} of the pork isolates and 11{\%} of the pig isolates. Of the 113 sul1 positive isolates, 97 carried the integron-associated integrase gene hall. All 20 sul3 positive isolates were positive for intl1, and in 12 of these isolates sul3 was the only sulphonamide resistance gene detected. The origin of sul1 and sul2 found in isolates from healthy humans is speculative, but their spread from pigs to humans via the food chain is possible.",
keywords = "food animals, resistance genes, antibiotic resistance, integrons",
author = "Hammerum, {Anette Marie} and Dorthe Sandvag and Andersen, {Sigrid R.} and Seyfarth, {Anne Mette} and Porsbo, {Lone Jannok} and Niels Frimodt-M{\o}ller and Heuer, {Ole Eske}",
year = "2006",
doi = "10.1016/j.ijfoodmicro.2005.06.023",
language = "English",
volume = "106",
pages = "235--237",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
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Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark. / Hammerum, Anette Marie; Sandvag, Dorthe; Andersen, Sigrid R.; Seyfarth, Anne Mette; Porsbo, Lone Jannok; Frimodt-Møller, Niels; Heuer, Ole Eske.

In: International Journal of Food Microbiology, Vol. 106, No. 2, 2006, p. 235-237.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark

AU - Hammerum, Anette Marie

AU - Sandvag, Dorthe

AU - Andersen, Sigrid R.

AU - Seyfarth, Anne Mette

AU - Porsbo, Lone Jannok

AU - Frimodt-Møller, Niels

AU - Heuer, Ole Eske

PY - 2006

Y1 - 2006

N2 - The occurrence of sulphonamide resistance was investigated in 998 Escherichia coli isolates, obtained from pig faeces collected at slaughter, Danish pork collected at retail outlets and from faeces from healthy persons in Denmark. In total 18% (n = 35), 20% (n = 38) and 26% (n = 161) of the E. coli isolates obtained from humans, pork and pigs, respectively, were resistant to sulphonamide. All sulphonamide resistant E. coli isolates were investigated for the presence of sul1, sul2, sul3 and intl1 genes by PCR. The sul1 gene was detected in 40% (n = 14), 29% (n = 11) and 55% (n = 88) of the sulphonamide resistant isolates from humans, pork and pigs, respectively. The sul2 gene was detected in 80% (n = 28), 76% (n = 29) and 50% (n = 81) of isolates from humans, pork and pigs, respectively. None of the human isolates were PCR-positive for sul3, whereas sul3 was present in 5% of the pork isolates and 11% of the pig isolates. Of the 113 sul1 positive isolates, 97 carried the integron-associated integrase gene hall. All 20 sul3 positive isolates were positive for intl1, and in 12 of these isolates sul3 was the only sulphonamide resistance gene detected. The origin of sul1 and sul2 found in isolates from healthy humans is speculative, but their spread from pigs to humans via the food chain is possible.

AB - The occurrence of sulphonamide resistance was investigated in 998 Escherichia coli isolates, obtained from pig faeces collected at slaughter, Danish pork collected at retail outlets and from faeces from healthy persons in Denmark. In total 18% (n = 35), 20% (n = 38) and 26% (n = 161) of the E. coli isolates obtained from humans, pork and pigs, respectively, were resistant to sulphonamide. All sulphonamide resistant E. coli isolates were investigated for the presence of sul1, sul2, sul3 and intl1 genes by PCR. The sul1 gene was detected in 40% (n = 14), 29% (n = 11) and 55% (n = 88) of the sulphonamide resistant isolates from humans, pork and pigs, respectively. The sul2 gene was detected in 80% (n = 28), 76% (n = 29) and 50% (n = 81) of isolates from humans, pork and pigs, respectively. None of the human isolates were PCR-positive for sul3, whereas sul3 was present in 5% of the pork isolates and 11% of the pig isolates. Of the 113 sul1 positive isolates, 97 carried the integron-associated integrase gene hall. All 20 sul3 positive isolates were positive for intl1, and in 12 of these isolates sul3 was the only sulphonamide resistance gene detected. The origin of sul1 and sul2 found in isolates from healthy humans is speculative, but their spread from pigs to humans via the food chain is possible.

KW - food animals

KW - resistance genes

KW - antibiotic resistance

KW - integrons

U2 - 10.1016/j.ijfoodmicro.2005.06.023

DO - 10.1016/j.ijfoodmicro.2005.06.023

M3 - Journal article

VL - 106

SP - 235

EP - 237

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 2

ER -