Abstract
| Original language | English |
|---|---|
| Journal | Procedia Technology |
| Volume | 27 |
| Pages (from-to) | 190-192 |
| Number of pages | 3 |
| ISSN | 2212-0173 |
| DOIs | |
| Publication status | Published - 2017 |
| Event | Biosensors 2016: 26th Anniversary World Congress on Biosensors - Swedish Exhibition and Congress Centre, Gothenburg, Sweden Duration: 25 May 2016 → 27 May 2016 Conference number: 26 http://www.biosensors-congress.elsevier.com/ |
Conference
| Conference | Biosensors 2016 |
|---|---|
| Number | 26 |
| Location | Swedish Exhibition and Congress Centre |
| Country | Sweden |
| City | Gothenburg |
| Period | 25/05/2016 → 27/05/2016 |
| Internet address |
Keywords
- Surface enchanced Raman spectroscopy
- P-coumaric acid
- E. coli
- Microbial factories
- Fast analysis
Cite this
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Detection of p-coumaric acid from cell supernatant using surface enhanced Raman scattering. / Morelli, Lidia; Jendresen, Christian Bille; Zor, Kinga; Rindzevicius, Tomas; Schmidt, Michael Stenbæk; Nielsen, Alex Toftgaard; Boisen, Anja.
In: Procedia Technology, Vol. 27, 2017, p. 190-192.Research output: Contribution to journal › Conference abstract in journal › Research › peer-review
TY - ABST
T1 - Detection of p-coumaric acid from cell supernatant using surface enhanced Raman scattering
AU - Morelli, Lidia
AU - Jendresen, Christian Bille
AU - Zor, Kinga
AU - Rindzevicius, Tomas
AU - Schmidt, Michael Stenbæk
AU - Nielsen, Alex Toftgaard
AU - Boisen, Anja
PY - 2017
Y1 - 2017
N2 - A standard protocol for analysis of microbial factories requires the screening of several populations in order to find the bestperforming ones. Standard analytical methods usually include high performance liquid chromatography (HPLC), thin layerchromatography (TLC) or spectrophotometry, which are expensive and time-consuming processes. Surface Enhanced Raman Spectroscopy (SERS), instead, is a highly sensitive spectroscopic technique for specific, fast and real-time sensing of biologicalsamples. Here we demonstrate the use of SERS to discriminate between two different bacterial populations based on detection ofp-coumaric acid (pHCA) in cell supernatant.SERS active substrates, based on leaning gold-capped silicon nanopillars, were used for detection. They were successfullyused to detect culture medium spiked with pHCA, and the effect of medium dilution was studied. For analysis of biologicalproduction of pHCA, triplicate cultures of E. coli strains expressing a pHCA-forming enzyme (P) as well as of a non-producingstrain (C) were grown. Then, supernatant samples were collected and their pHCA content was measured using SERS and HPLCfor comparison. The intensity of the pHCA Raman mode at 1169 cm-1 (CH-rocking motion) showed different trends for P and Cstrains, similar to the results obtained using the HPLC method. Results illustrate that SERS can be used for quick and semiquantitativediscrimination of pHCA concentrations in cell supernatant medium.
AB - A standard protocol for analysis of microbial factories requires the screening of several populations in order to find the bestperforming ones. Standard analytical methods usually include high performance liquid chromatography (HPLC), thin layerchromatography (TLC) or spectrophotometry, which are expensive and time-consuming processes. Surface Enhanced Raman Spectroscopy (SERS), instead, is a highly sensitive spectroscopic technique for specific, fast and real-time sensing of biologicalsamples. Here we demonstrate the use of SERS to discriminate between two different bacterial populations based on detection ofp-coumaric acid (pHCA) in cell supernatant.SERS active substrates, based on leaning gold-capped silicon nanopillars, were used for detection. They were successfullyused to detect culture medium spiked with pHCA, and the effect of medium dilution was studied. For analysis of biologicalproduction of pHCA, triplicate cultures of E. coli strains expressing a pHCA-forming enzyme (P) as well as of a non-producingstrain (C) were grown. Then, supernatant samples were collected and their pHCA content was measured using SERS and HPLCfor comparison. The intensity of the pHCA Raman mode at 1169 cm-1 (CH-rocking motion) showed different trends for P and Cstrains, similar to the results obtained using the HPLC method. Results illustrate that SERS can be used for quick and semiquantitativediscrimination of pHCA concentrations in cell supernatant medium.
KW - Surface enchanced Raman spectroscopy
KW - P-coumaric acid
KW - E. coli
KW - Microbial factories
KW - Fast analysis
U2 - 10.1016/j.protcy.2017.04.081
DO - 10.1016/j.protcy.2017.04.081
M3 - Conference abstract in journal
VL - 27
SP - 190
EP - 192
JO - Procedia Technology
JF - Procedia Technology
SN - 2212-0173
ER -