Detection of Mycobacterium avium subsp. paratuberculosis in Milk from Clinically Affected Cows by PCR and culture

Steen Bjørck Giese, Peter Ahrens

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    Milk and faecal samples from cows with clinical symptoms of paratuberculosis were examined for the presence of Mycobacterium avium subsp.paratuberculosis (M. a. paratuberculosis) by culture and PCR. M. a. paratuberculosis was isolated in varied numbers from faeces or intestinal mucosa in 8 of 11 animals. In milk from 5 cows (all faecal culture-positive) we cultivated a few colonies of M. a. paratuberculosis (less than 100 CFU per mi). Milk samples from 2 cows were PCR-positive (both animals were faecal culture-positive, and 1 cow was milk culture positive). One cow was culture-negative on intestinal mucosa, but culture-positive in milk, and both faeces and milk were negative in culture and PCR from 2 cows. In conclusion the presence of M. a. paratuberculosis could be detected in raw milk by PCR but cultivation of milk was more sensitive in detecting the organism.
    Original languageEnglish
    Title of host publicationProceedings of the 6th International Colloquium on Paratuberculosis
    PublisherInternational Association of Paratuberculosis inc.
    Publication date1999
    Pages553-558
    ISBN (Print)0-9633043-4-8
    Publication statusPublished - 1999
    Event6th International Colloquium on Paratuberculosis - Melbourne, Australia
    Duration: 14 Feb 199918 Feb 1999
    Conference number: 6

    Conference

    Conference6th International Colloquium on Paratuberculosis
    Number6
    CountryAustralia
    CityMelbourne
    Period14/02/199918/02/1999

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