Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture

Steen Bjørck Giese, Peter Ahrens

    Research output: Contribution to journalConference articleResearchpeer-review

    Abstract

    Milk and faeces samples from cows with clinical symptoms of paratuberculosis were examined for the presence of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) by culture and PCR. M. paratuberculosis was cultivated in variable numbers from faeces or intestinal mucosa in eight of 11 animals. In milk from five cows (all faeces culture positive), we cultivated a few colonies of M. paratuberculosis (<100 CFU per ml). Milk samples from two cows were PCR positive (both animals were faeces culture positive, and one cow was milk culture positive). One cow was culture negative on intestinal mucosa, but culture positive in milk, and two cows were negative in culture and PCR from both faeces and milk. In conclusion, the presence of M. paratuberculosis could be detected in raw milk by PCR, but cultivation of milk was more sensitive. (C) 2000 Elsevier Science B.V. All rights reserved.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume77
    Issue number3-4
    Pages (from-to)291-297
    ISSN0378-1135
    DOIs
    Publication statusPublished - 20 Dec 2000
    Event6th International Colloquium on Paratuberculosis - Melbourne, Australia
    Duration: 14 Feb 199918 Feb 1999
    Conference number: 6

    Conference

    Conference6th International Colloquium on Paratuberculosis
    Number6
    CountryAustralia
    CityMelbourne
    Period14/02/199918/02/1999

    Keywords

    • mycobacterium paratuberculosis
    • milk
    • culture
    • PCR

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