Milk and faeces samples from cows with clinical symptoms of paratuberculosis were examined for the presence of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) by culture and PCR. M. paratuberculosis was cultivated in variable numbers from faeces or intestinal mucosa in eight of 11 animals. In milk from five cows (all faeces culture positive), we cultivated a few colonies of M. paratuberculosis (<100 CFU per ml). Milk samples from two cows were PCR positive (both animals were faeces culture positive, and one cow was milk culture positive). One cow was culture negative on intestinal mucosa, but culture positive in milk, and two cows were negative in culture and PCR from both faeces and milk. In conclusion, the presence of M. paratuberculosis could be detected in raw milk by PCR, but cultivation of milk was more sensitive. (C) 2000 Elsevier Science B.V. All rights reserved.
|Publication status||Published - 20 Dec 2000|
|Event||6th International Colloquium on Paratuberculosis - Melbourne, Australia|
Duration: 14 Feb 1999 → 18 Feb 1999
Conference number: 6
|Conference||6th International Colloquium on Paratuberculosis|
|Period||14/02/1999 → 18/02/1999|
- mycobacterium paratuberculosis