Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

Research output: Contribution to journalJournal article – Annual report year: 2007Researchpeer-review

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Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. / Skottrup, Peter; Hearty, Stephen; Frøkiær, Hanne; Leonard, Paul; Hejgaard, Jørn; O'Kennedy, Richard; Nicolaisen, Mogens; Fejer Justesen, Annemarie.

In: Biosensors and Bioelectronics, Vol. 22, No. 11, 2007, p. 2724-2729.

Research output: Contribution to journalJournal article – Annual report year: 2007Researchpeer-review

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Skottrup, Peter ; Hearty, Stephen ; Frøkiær, Hanne ; Leonard, Paul ; Hejgaard, Jørn ; O'Kennedy, Richard ; Nicolaisen, Mogens ; Fejer Justesen, Annemarie. / Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. In: Biosensors and Bioelectronics. 2007 ; Vol. 22, No. 11. pp. 2724-2729.

Bibtex

@article{a72cb6ae88824999a53f27002da3d185,
title = "Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay",
abstract = "This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.",
author = "Peter Skottrup and Stephen Hearty and Hanne Fr{\o}ki{\ae}r and Paul Leonard and J{\o}rn Hejgaard and Richard O'Kennedy and Mogens Nicolaisen and {Fejer Justesen}, Annemarie",
year = "2007",
doi = "10.1016/j.bios.2006.11.017",
language = "English",
volume = "22",
pages = "2724--2729",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier",
number = "11",

}

RIS

TY - JOUR

T1 - Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

AU - Skottrup, Peter

AU - Hearty, Stephen

AU - Frøkiær, Hanne

AU - Leonard, Paul

AU - Hejgaard, Jørn

AU - O'Kennedy, Richard

AU - Nicolaisen, Mogens

AU - Fejer Justesen, Annemarie

PY - 2007

Y1 - 2007

N2 - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

AB - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

U2 - 10.1016/j.bios.2006.11.017

DO - 10.1016/j.bios.2006.11.017

M3 - Journal article

VL - 22

SP - 2724

EP - 2729

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 11

ER -