Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

Peter Skottrup; Stephen Hearty; Hanne Frøkiær; Paul Leonard; Jørn Hejgaard; Richard O'Kennedy; Mogens Nicolaisen; Annemarie Fejer Justesen

doi:10.1016/j.bios.2006.11.017

Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O'Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

Original language	English
Journal	Biosensors and Bioelectronics
Volume	22
Issue number	11
Pages (from-to)	2724-2729
ISSN	0956-5663
DOIs	https://doi.org/10.1016/j.bios.2006.11.017
Publication status	Published - 2007

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title = "Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay",

abstract = "This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.",

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T1 - Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

AU - Skottrup, Peter

AU - Hearty, Stephen

AU - Frøkiær, Hanne

AU - Leonard, Paul

AU - Hejgaard, Jørn

AU - O'Kennedy, Richard

AU - Nicolaisen, Mogens

AU - Fejer Justesen, Annemarie

PY - 2007

Y1 - 2007

N2 - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

AB - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

U2 - 10.1016/j.bios.2006.11.017

DO - 10.1016/j.bios.2006.11.017

M3 - Journal article

SN - 0956-5663

VL - 22

SP - 2724

EP - 2729

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 11

ER -

Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

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