Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

Peter Skottrup, Stephen Hearty, Hanne Frøkiær, Paul Leonard, Jørn Hejgaard, Richard O'Kennedy, Mogens Nicolaisen, Annemarie Fejer Justesen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.
    Original languageEnglish
    JournalBiosensors and Bioelectronics
    Volume22
    Issue number11
    Pages (from-to)2724-2729
    ISSN0956-5663
    DOIs
    Publication statusPublished - 2007

    Cite this

    Skottrup, P., Hearty, S., Frøkiær, H., Leonard, P., Hejgaard, J., O'Kennedy, R., ... Fejer Justesen, A. (2007). Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. Biosensors and Bioelectronics, 22(11), 2724-2729. https://doi.org/10.1016/j.bios.2006.11.017
    Skottrup, Peter ; Hearty, Stephen ; Frøkiær, Hanne ; Leonard, Paul ; Hejgaard, Jørn ; O'Kennedy, Richard ; Nicolaisen, Mogens ; Fejer Justesen, Annemarie. / Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. In: Biosensors and Bioelectronics. 2007 ; Vol. 22, No. 11. pp. 2724-2729.
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    title = "Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay",
    abstract = "This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.",
    author = "Peter Skottrup and Stephen Hearty and Hanne Fr{\o}ki{\ae}r and Paul Leonard and J{\o}rn Hejgaard and Richard O'Kennedy and Mogens Nicolaisen and {Fejer Justesen}, Annemarie",
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    Skottrup, P, Hearty, S, Frøkiær, H, Leonard, P, Hejgaard, J, O'Kennedy, R, Nicolaisen, M & Fejer Justesen, A 2007, 'Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay', Biosensors and Bioelectronics, vol. 22, no. 11, pp. 2724-2729. https://doi.org/10.1016/j.bios.2006.11.017

    Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay. / Skottrup, Peter; Hearty, Stephen; Frøkiær, Hanne; Leonard, Paul; Hejgaard, Jørn; O'Kennedy, Richard; Nicolaisen, Mogens; Fejer Justesen, Annemarie.

    In: Biosensors and Bioelectronics, Vol. 22, No. 11, 2007, p. 2724-2729.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Detection of Fungal Spores Using a Generic Surface Plasmon Resonance Immunoassay

    AU - Skottrup, Peter

    AU - Hearty, Stephen

    AU - Frøkiær, Hanne

    AU - Leonard, Paul

    AU - Hejgaard, Jørn

    AU - O'Kennedy, Richard

    AU - Nicolaisen, Mogens

    AU - Fejer Justesen, Annemarie

    PY - 2007

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    N2 - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

    AB - This paper describes a biosensor-based method for detection of fungal spores using Surface Plasmon Resonance (SPR). The approach involves the use of a mouse monoclonal antibody (Pst mAb8) and a SPR sensor for label-free detection of urediniospores from the model organism Puccinia striiformis f.sp. tritici (Pst). In the subtractive inhibition assay, urediniospores and Pst mAb8 were mixed, urediniospore-bound Pst mAb8 removed by centrifugation and the remaining Pst mAb8 quantified using the SPR sensor. Assay conditions were optimised and a detection limit of 3.1 x 105 urediniospores/ml was achieved. Spiked Pst samples were further examined in a background of a related spore and it was found that Pst quantification was possible in this mixture. This study represent the first use of SPR technology for fungal spore detection as well as the first report of a successful biosensor-based detection strategy for Pst.

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    DO - 10.1016/j.bios.2006.11.017

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    JO - Biosensors and Bioelectronics

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    ER -