Detection of Campylobacter Bacteria in Air Samples for Continuous Real-Time Monitoring of Campylobacter Colonization in Broiler Flocks

Katja Nyholm Olsen, Marianne Lund, J. Skov, Laurids Siig Christensen, Jeffrey Hoorfar

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Improved monitoring tools are important for the control of Campylobacter bacteria in broiler production. In this study, we compare the sensitivities of detection of Campylobacter by PCR with feces, dust, and air samples during the lifetimes of broilers in two poultry houses and conclude that the sensitivity of detection of Campylobacter in air is comparable to that in other sample materials. Profiling of airborne particles in six poultry houses revealed that the aerodynamic conditions were dependent on the age of the chickens and very comparable among different poultry houses, with low proportions of particles in the 0.5- to 2-mu m-diameter range and high proportions in the 2- to 5-mu m-diameter range. Campylobacter could also be detected by PCR in air samples collected at the hanging stage during the slaughter process but not at the other stages tested at the slaughterhouse. The exploitation of airborne dust in poultry houses as a sample material for the detection of Campylobacter and other pathogens provides an intriguing possibility, in conjunction with new detection technologies, for allowing continuous or semicontinuous monitoring of colonization status.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume75
Issue number7
Pages (from-to)2074-2078
ISSN0099-2240
DOIs
Publication statusPublished - 2009

Fingerprint Dive into the research topics of 'Detection of Campylobacter Bacteria in Air Samples for Continuous Real-Time Monitoring of Campylobacter Colonization in Broiler Flocks'. Together they form a unique fingerprint.

Cite this