Detection of antibodies against Actinobacillus pleuropneumoniae serotype 5 using an inhibition enzyme immunoassay

Eva I. Stenbæk, F. DeLaSalle, M. Gottschalk

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    An inhibition enzyme immunoassay (EIA) for detection of antibodies against A. pleuropneumoniae serotype 5 (App-5) in pig sera, based on the inhibition of the binding of an App-5 specific monoclonal antibody was established. The monoclonal antibody (MAb 210-F11) was found to be directed against an epitope on the O-chain of App-5 LPS, In the inhibition EIA, highly purified App-5 LPS was used to coat microtitre plates, Serial dilutions of pig sera were added to the plates prior to the addition of the MAb 210-F11. The degree of binding of App-5 antibodies from pig sera was determined as the percentage inhibition of the MAb 210-F11. Pig serum from specific pathogen free (SPF) herds, from experimentally infected animals, and from acutely and chronically infected herds were tested, A serum dilution of 1/30 was found to be optimal, when using 50% inhibition as the discriminating inhibition percentage. No cross-reactivity was observed with serum from pigs infected with other App serotypes or bacteria isolated from the respiratory tract, such as A. suis and H. parasuis. The inhibition EIA will be used for surviellance of App-5 antibodies in SPF and conventional herds.
    Original languageEnglish
    JournalCanadian Journal of Veterinary Research-Revue Canadienne de Recherche Veterinaire
    Volume61
    Issue number1
    Pages (from-to)1-7
    ISSN0830-9000
    Publication statusPublished - 1997

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