Detection of analyte binding to microarrays using gold nanoparticle labels and a desktop scanner

Anpan Han, Martin Dufva, Erik Belleville, Claus Bo Vöge Christensen

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Microarray hybridization or antibody binding can be detected by many techniques, however, only a few are suitable for widespread use since many of these detection techniques rely on bulky and expensive instruments. Here, we describe the usefulness of a simple and inexpensive detection method based on gold nanoparticle labeled antibodies visualized by a commercial, office desktop flatbed scanner. Scanning electron microscopy studies showed that the signal from the flatbed scanner was proportional to the surface density of the bound antibody-gold conjugates, and that the flatbed scanner could detect six attomoles of antibody-gold conjugates. This detection system was used in a competitive immunoassay to measure the concentration of the pesticide metabolite 2,6-dichlorobenzamide (BAM) in water samples. The results showed that the gold labeled antibodies functioned comparably with a fluorescent based immunoassay for detecting BAM in water. A qualitative immunoassay based on gold-labeled antibodies could determineif a water sample contained BAM above and below 60-70 ng L(-1), which is below the maximum allowed BAM concentration for drinking water (100 ng L(-1)) according to European Union legislation.
    Original languageEnglish
    JournalLab on a Chip
    Volume3
    Issue number4
    Pages (from-to)329-332
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2003

    Bibliographical note

    © The Royal Society of Chemistry 2003

    Keywords

    • Antibodies, Monoclonal
    • Buffers
    • Fluorescent Antibody Technique
    • Gold
    • Immunohistochemistry
    • Indicators and Reagents
    • Microspheres
    • Pesticide Residues
    • Protein Array Analysis
    • Water Pollutants, Chemical

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