Detection of an Actinobacillus pleuropneumoniae serotype 2 lipopolysaccharide (LPS) variant

E.I. Stenbaek, K. HovindHaugen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Until now 12 serotypes of Actinobacillus pleuropneumoniae have been recognized. The specificity of the serotypes reside in the carbohydrate composition of the capsular polysaccharides and lipopolysaccharides (LPS). The LPS of A. pleuropneumoniae serotype 2 is a smooth type LPS with O-chains of linear repeating pentasaccharide units with an O-acetyl group linked to a glucose unit. A monoclonal antibody (MAb 102-G02) directed against A. pleuropneumoniae serotype 2 was characterized in enzyme linked immunosorbent assay (ELISA) and in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The MAI, 102-G02 was directed against an epitope on the O-chain of the LPS and was used to define a new LPS variant of A. pleuropneumoniae serotype 2 (referred to as A. pleuropneumoniae serotype 2X). Investigation of the reactivity of the MAb 102-G02 against an A. pleuropneumoniae serotype 2X field isolate (9008) and the Danish App-2 strain 4226 in electron microscopy, confirmed the different binding patterns.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume52
    Issue number3-4
    Pages (from-to)285-292
    ISSN0378-1135
    DOIs
    Publication statusPublished - 1996

    Keywords

    • Actinobacillus pleuropneumoniae
    • lipopolysaccharides
    • monoclonal antibodies

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