Detection of 3-hydroxykynurenine in a plant pathogenic fungus

Jens Øllgaard Duus, Richard P. Oliver, Karl Kristian Thomsen, T. J. Greer Wilson, Bent O. Petersen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A redox-active compound has been purified from the barley powdery mildew fungus Blumeria (Erysiphe) graminis f. sp. hordei. A combination of spectrophotometry, MS and NMR has identified it as 3-hydroxykynurenine (3OHKyn). This compound, never previously detected in any fungus or pathogen, is best known for its role in vertebrate cataracts. It is found abundantly in developing and germinating spores and also in runner hyphae. Two roles for 3OHKyn are discussed: first, the presence of active oxygen species would enable 3OHKyn to cross-link the spore chemically with the plant. Secondly, it may be acting as an UV protectant and an antioxidant.
Original languageEnglish
JournalBiochemical Journal
Volume371
Issue number3
Pages (from-to)783-788
Number of pages6
ISSN0264-6021
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • Antioxidants
  • Fungi
  • Oxygen
  • Pathology
  • Plants (botany)
  • Biochemistry
  • 3 hydroxykynurenine
  • antioxidant
  • oxygen derivative
  • sunscreen
  • article
  • Blumeria graminis
  • cataract
  • cross linking
  • drug determination
  • drug purification
  • fungus
  • fungus hyphae
  • fungus spore
  • matrix assisted laser desorption ionization time of flight mass spectrometry
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • oxidation reduction reaction
  • priority journal
  • spectrophotometry
  • spore germination
  • ultraviolet radiation
  • Ascomycota
  • Kynurenine
  • Mass Spectrometry
  • Plants
  • Blumeria
  • Blumeria graminis f. sp. hordei
  • Erysiphales
  • Hordeum vulgare subsp. vulgare
  • Vertebrata
  • Pathogenic fungus
  • Ferric reduction
  • Hydroxykynurenine
  • T
  • X
  • Chordata Animalia (Animals, Chordates, Nonhuman Vertebrates, Vertebrates) - Vertebrata [85150] vertebrate common
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Blumeria graminis f. sp. hordei form powdery mildew fungus common pathogen
  • 3-hydroxykynurenine 484-78-6 UV protectant, antioxidant, detection, identification, redox-active compound
  • active oxygen species
  • ferric reductase 62168-78-9
  • iron 7439-89-6 acquisition, utilization
  • 06502, Radiation biology - General
  • 10069, Biochemistry studies - Minerals
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 16504, Reproductive system - Physiology and biochemistry
  • 20004, Sense organs - Physiology and biochemistry
  • 20006, Sense organs - Pathology
  • 25502, Development and Embryology - General and descriptive
  • 51510, Plant physiology - Growth, differentiation
  • 51512, Plant physiology - Reproduction
  • 51516, Plant physiology - Light and radiation effects
  • 51518, Plant physiology - Enzymes
  • Enzymology
  • Infection
  • Methods and Techniques
  • Radiation Biology
  • Sense Organs
  • cataract Cataract (MeSH) eye disease etiology
  • pathogen-plant interaction
  • UV radiation
  • Biochemistry and Molecular Biophysics
  • Sensory Reception
  • runner hyphae development
  • spore reproductive system development, germination
  • mass spectrometry laboratory techniques, spectrum analysis techniques
  • NMR laboratory techniques, spectrum analysis techniques
  • spectrophotometry laboratory techniques, spectrum analysis techniques

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