Detection and quantification of Flavobacterium psychrophilum specific bacteriophages in rainbow trout upon different administration methods: Implications for disease control in aquaculture

Rói Hammershaimb Christiansen, Lone Madsen, Inger Dalsgaard, A. Middelboe

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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    Abstract

    Flavobacterium psychrophilum is the pathogen causing the disease rainbow trout fry syndrome (RTFS), which has important implications for aquaculture production and trade worldwide. RTFS can be treated by antibiotic administration, but with the increasing problem of antibiotic resistant bacteria, the use of lytic bacteriophages is a promising alternative approach to disease control in aquaculture. Bacteriophage control of bacterial infections depends on efficient delivery of the phages to the infected organs, and in this study we therefore examined the occurrence and persistence of phages in the internal organs in rainbow trout, following different administration methods. Three phage administration methods using phage FpV-9 were used: phage bath, oral administration of phagesuspension directly into the stomach and feeding with phage-coated feed pellets. Phages were detected in all the four examined organs (intestine, brain, spleen and liver) with all three administration methods, demonstrating that the phages are capable of passing the intestinal wall and entering the bloodstream. The highest phage concentration was found in the intestine where a maximum of 3x1010 phages g-1 was obtained after oral administration of phage-suspension, but also phage addition via phage-coated feed pellets resulted in high phage titers (5x106 phages g-1 intestine). The concentration of phages in the spleen was 100 fold lower than in the intestine, suggesting a large phage decay during transport to the inner organs. These results provide the basis for future phage treatment of RTFS.
    Original languageEnglish
    Title of host publicationDAFINET Workshop and Ph.D. Course : Book of abstracts
    Number of pages1
    PublisherDAFINET
    Publication date2013
    Publication statusPublished - 2013
    EventDAFINET Workshop : Diagnosis and Control of Fish Diseases - Frederiksberg, Denmark
    Duration: 9 Apr 201311 Apr 2013
    http://www.dafinet.dk

    Conference

    ConferenceDAFINET Workshop : Diagnosis and Control of Fish Diseases
    CountryDenmark
    CityFrederiksberg
    Period09/04/201311/04/2013
    Internet address

    Cite this

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    title = "Detection and quantification of Flavobacterium psychrophilum specific bacteriophages in rainbow trout upon different administration methods: Implications for disease control in aquaculture",
    abstract = "Flavobacterium psychrophilum is the pathogen causing the disease rainbow trout fry syndrome (RTFS), which has important implications for aquaculture production and trade worldwide. RTFS can be treated by antibiotic administration, but with the increasing problem of antibiotic resistant bacteria, the use of lytic bacteriophages is a promising alternative approach to disease control in aquaculture. Bacteriophage control of bacterial infections depends on efficient delivery of the phages to the infected organs, and in this study we therefore examined the occurrence and persistence of phages in the internal organs in rainbow trout, following different administration methods. Three phage administration methods using phage FpV-9 were used: phage bath, oral administration of phagesuspension directly into the stomach and feeding with phage-coated feed pellets. Phages were detected in all the four examined organs (intestine, brain, spleen and liver) with all three administration methods, demonstrating that the phages are capable of passing the intestinal wall and entering the bloodstream. The highest phage concentration was found in the intestine where a maximum of 3x1010 phages g-1 was obtained after oral administration of phage-suspension, but also phage addition via phage-coated feed pellets resulted in high phage titers (5x106 phages g-1 intestine). The concentration of phages in the spleen was 100 fold lower than in the intestine, suggesting a large phage decay during transport to the inner organs. These results provide the basis for future phage treatment of RTFS.",
    author = "Christiansen, {R{\'o}i Hammershaimb} and Lone Madsen and Inger Dalsgaard and A. Middelboe",
    year = "2013",
    language = "English",
    booktitle = "DAFINET Workshop and Ph.D. Course",
    publisher = "DAFINET",

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    Christiansen, RH, Madsen, L, Dalsgaard, I & Middelboe, A 2013, Detection and quantification of Flavobacterium psychrophilum specific bacteriophages in rainbow trout upon different administration methods: Implications for disease control in aquaculture. in DAFINET Workshop and Ph.D. Course: Book of abstracts. DAFINET, DAFINET Workshop : Diagnosis and Control of Fish Diseases, Frederiksberg, Denmark, 09/04/2013.

    Detection and quantification of Flavobacterium psychrophilum specific bacteriophages in rainbow trout upon different administration methods: Implications for disease control in aquaculture. / Christiansen, Rói Hammershaimb; Madsen, Lone; Dalsgaard, Inger; Middelboe, A.

    DAFINET Workshop and Ph.D. Course: Book of abstracts. DAFINET, 2013.

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    TY - ABST

    T1 - Detection and quantification of Flavobacterium psychrophilum specific bacteriophages in rainbow trout upon different administration methods: Implications for disease control in aquaculture

    AU - Christiansen, Rói Hammershaimb

    AU - Madsen, Lone

    AU - Dalsgaard, Inger

    AU - Middelboe, A.

    PY - 2013

    Y1 - 2013

    N2 - Flavobacterium psychrophilum is the pathogen causing the disease rainbow trout fry syndrome (RTFS), which has important implications for aquaculture production and trade worldwide. RTFS can be treated by antibiotic administration, but with the increasing problem of antibiotic resistant bacteria, the use of lytic bacteriophages is a promising alternative approach to disease control in aquaculture. Bacteriophage control of bacterial infections depends on efficient delivery of the phages to the infected organs, and in this study we therefore examined the occurrence and persistence of phages in the internal organs in rainbow trout, following different administration methods. Three phage administration methods using phage FpV-9 were used: phage bath, oral administration of phagesuspension directly into the stomach and feeding with phage-coated feed pellets. Phages were detected in all the four examined organs (intestine, brain, spleen and liver) with all three administration methods, demonstrating that the phages are capable of passing the intestinal wall and entering the bloodstream. The highest phage concentration was found in the intestine where a maximum of 3x1010 phages g-1 was obtained after oral administration of phage-suspension, but also phage addition via phage-coated feed pellets resulted in high phage titers (5x106 phages g-1 intestine). The concentration of phages in the spleen was 100 fold lower than in the intestine, suggesting a large phage decay during transport to the inner organs. These results provide the basis for future phage treatment of RTFS.

    AB - Flavobacterium psychrophilum is the pathogen causing the disease rainbow trout fry syndrome (RTFS), which has important implications for aquaculture production and trade worldwide. RTFS can be treated by antibiotic administration, but with the increasing problem of antibiotic resistant bacteria, the use of lytic bacteriophages is a promising alternative approach to disease control in aquaculture. Bacteriophage control of bacterial infections depends on efficient delivery of the phages to the infected organs, and in this study we therefore examined the occurrence and persistence of phages in the internal organs in rainbow trout, following different administration methods. Three phage administration methods using phage FpV-9 were used: phage bath, oral administration of phagesuspension directly into the stomach and feeding with phage-coated feed pellets. Phages were detected in all the four examined organs (intestine, brain, spleen and liver) with all three administration methods, demonstrating that the phages are capable of passing the intestinal wall and entering the bloodstream. The highest phage concentration was found in the intestine where a maximum of 3x1010 phages g-1 was obtained after oral administration of phage-suspension, but also phage addition via phage-coated feed pellets resulted in high phage titers (5x106 phages g-1 intestine). The concentration of phages in the spleen was 100 fold lower than in the intestine, suggesting a large phage decay during transport to the inner organs. These results provide the basis for future phage treatment of RTFS.

    M3 - Conference abstract in proceedings

    BT - DAFINET Workshop and Ph.D. Course

    PB - DAFINET

    ER -