Detection and identification of Actinobacillus pleuropneumoniae serotypes 1, 2, and 8 by multiplex PCR

J.A. Schuchert, T.J. Inzana, Øystein Angen, S. Jessing

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.
    Original languageEnglish
    JournalJournal of Clinical Microbiology
    Volume42
    Issue number9
    Pages (from-to)4344-4348
    ISSN0095-1137
    DOIs
    Publication statusPublished - 2004

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