Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Christina M. Lazov; Mariann Chriél; Hans J. Baagøe; Esben Fjederholt; Yu Deng; Engbert A. Kooi; Graham J. Belsham; Anette Bøtner; Thomas Bruun Rasmussen

doi:10.3390/v10090486

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Christina M. Lazov, Mariann Chriél, Hans J. Baagøe, Esben Fjederholt, Yu Deng, Engbert A. Kooi, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

312 Downloads (Pure)

Abstract

Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

Original language	English
Article number	486
Journal	Viruses
Volume	10
Issue number	9
Number of pages	13
ISSN	1999-4915
DOIs	https://doi.org/10.3390/v10090486
Publication status	Published - 2018

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Keywords

Europe
Vespertilionidae
Coronavirus
Host restriction
Nucleotide sequencing
Phylogenetic analysis
Microbiology

Access to Document

10.3390/v10090486

Viruses 10 00486Final published version, 2.27 MB

OpenUrl availability

Full text

Cite this

@article{f7b780fbefc747b4bc8a24be5c01ef11,

title = "Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark",

abstract = "Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.",

keywords = "Europe, Vespertilionidae, Coronavirus, Host restriction, Nucleotide sequencing, Phylogenetic analysis, Microbiology",

author = "Lazov, {Christina M.} and Mariann Chri{\'e}l and Baag{\o}e, {Hans J.} and Esben Fjederholt and Yu Deng and Kooi, {Engbert A.} and Belsham, {Graham J.} and Anette B{\o}tner and Rasmussen, {Thomas Bruun}",

note = "This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).",

year = "2018",

doi = "10.3390/v10090486",

language = "English",

volume = "10",

journal = "Viruses",

issn = "1999-4915",

publisher = "M D P I AG",

number = "9",

}

TY - JOUR

T1 - Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

AU - Lazov, Christina M.

AU - Chriél, Mariann

AU - Baagøe, Hans J.

AU - Fjederholt, Esben

AU - Deng, Yu

AU - Kooi, Engbert A.

AU - Belsham, Graham J.

AU - Bøtner, Anette

AU - Rasmussen, Thomas Bruun

N1 - This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PY - 2018

Y1 - 2018

N2 - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

AB - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

KW - Europe

KW - Vespertilionidae

KW - Coronavirus

KW - Host restriction

KW - Nucleotide sequencing

KW - Phylogenetic analysis

KW - Microbiology

U2 - 10.3390/v10090486

DO - 10.3390/v10090486

M3 - Journal article

C2 - 30208582

SN - 1999-4915

VL - 10

JO - Viruses

JF - Viruses

IS - 9

M1 - 486

ER -

Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Abstract

Bibliographical note

Keywords

Access to Document

OpenUrl availability

Fingerprint

Cite this