Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

Christina M. Lazov, Mariann Chriél, Hans J. Baagøe, Esben Fjederholt, Yu Deng, Engbert A. Kooi, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.
    Original languageEnglish
    Article number486
    JournalViruses
    Volume10
    Issue number9
    Number of pages13
    ISSN1999-4915
    DOIs
    Publication statusPublished - 2018

    Bibliographical note

    This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

    Keywords

    • Europe
    • Vespertilionidae
    • Coronavirus
    • Host restriction
    • Nucleotide sequencing
    • Phylogenetic analysis
    • Microbiology

    Cite this

    Lazov, C. M., Chriél, M., Baagøe, H. J., Fjederholt, E., Deng, Y., Kooi, E. A., ... Rasmussen, T. B. (2018). Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark. Viruses, 10(9), [486]. https://doi.org/10.3390/v10090486
    Lazov, Christina M. ; Chriél, Mariann ; Baagøe, Hans J. ; Fjederholt, Esben ; Deng, Yu ; Kooi, Engbert A. ; Belsham, Graham J. ; Bøtner, Anette ; Rasmussen, Thomas Bruun. / Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark. In: Viruses. 2018 ; Vol. 10, No. 9.
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    title = "Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark",
    abstract = "Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.",
    keywords = "Europe, Vespertilionidae, Coronavirus, Host restriction, Nucleotide sequencing, Phylogenetic analysis, Microbiology",
    author = "Lazov, {Christina M.} and Mariann Chri{\'e}l and Baag{\o}e, {Hans J.} and Esben Fjederholt and Yu Deng and Kooi, {Engbert A.} and Belsham, {Graham J.} and Anette B{\o}tner and Rasmussen, {Thomas Bruun}",
    note = "This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).",
    year = "2018",
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    Lazov, CM, Chriél, M, Baagøe, HJ, Fjederholt, E, Deng, Y, Kooi, EA, Belsham, GJ, Bøtner, A & Rasmussen, TB 2018, 'Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark', Viruses, vol. 10, no. 9, 486. https://doi.org/10.3390/v10090486

    Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark. / Lazov, Christina M.; Chriél, Mariann; Baagøe, Hans J.; Fjederholt, Esben; Deng, Yu; Kooi, Engbert A.; Belsham, Graham J.; Bøtner, Anette; Rasmussen, Thomas Bruun.

    In: Viruses, Vol. 10, No. 9, 486, 2018.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Detection and Characterization of Distinct Alphacoronaviruses in Five Different Bat Species in Denmark

    AU - Lazov, Christina M.

    AU - Chriél, Mariann

    AU - Baagøe, Hans J.

    AU - Fjederholt, Esben

    AU - Deng, Yu

    AU - Kooi, Engbert A.

    AU - Belsham, Graham J.

    AU - Bøtner, Anette

    AU - Rasmussen, Thomas Bruun

    N1 - This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

    PY - 2018

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    N2 - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

    AB - Bat populations harbour a multitude of viruses; some of these are pathogenic or potentially pathogenic in other animals or humans. Therefore, it is important to monitor the populations and characterize these viruses. In this study, the presence of coronaviruses (CoVs) in different species of Danish bats was investigated using active surveillance at different geographical locations in Denmark. Faecal samples were screened for the presence of CoVs using pan-CoV real-time RT-PCR assays. The amplicons, obtained from five different species of bats, were sequenced. Phylogenetic analysis revealed a species-specific clustering with the samples from Myotis daubentonii, showing a close resemblance to coronavirus sequences obtained from the same species of bat in Germany and the United Kingdom. Our results show, for the first time, that multiple, distinct alphacoronaviruses are present in the Danish bat populations.

    KW - Europe

    KW - Vespertilionidae

    KW - Coronavirus

    KW - Host restriction

    KW - Nucleotide sequencing

    KW - Phylogenetic analysis

    KW - Microbiology

    U2 - 10.3390/v10090486

    DO - 10.3390/v10090486

    M3 - Journal article

    C2 - 30208582

    VL - 10

    JO - Viruses

    JF - Viruses

    SN - 1999-4915

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    ER -