Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0

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Abstract

CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly sidestep these issues. The recently published CRISPR-BEST base editor system for actinobacteria is built around a C to T or A to G base exchange. These base editing systems however require additional constraints to be considered for designing the sgRNAs. Here, we present an updated version of the interactive CRISPy-web single guide RNA design tool https://crispy.secondarymetabolites.org/that was built to support “classical” CRISPR and now also CRISPR-BEST workflows.
Original languageEnglish
JournalSynthetic and Systems Biotechnology
Volume5
Issue number2
Pages (from-to)99-102
ISSN2405-805x
DOIs
Publication statusPublished - 2020

Keywords

  • CRISPR
  • Base editor
  • sgRNA
  • Webserver
  • CRISPR-BEST
  • Genome editing

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