Design of homo-organic acid producing strains using multi-objective optimization

Tae Yong Kim, Jong Myoung Park, Hyun Uk Kim, Kwang Myung Cho, Sang Yup Lee

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Production of homo-organic acids without byproducts is an important challenge in bioprocess engineering to minimize operation cost for separation processes. In this study, we used multi-objective optimization to design Escherichia coli strains with the goals of maximally producing target organic acids, while maintaining sufficiently high growth rate and minimizing the secretion of undesired byproducts. Homo-productions of acetic, lactic and succinic acids were targeted as examples. Engineered E. coli strains capable of producing homo-acetic and homo-lactic acids could be developed by taking this systems approach for the minimal identification of gene knockout targets. Also, failure to predict effective gene knockout targets for the homo-succinic acid production suggests that the multi-objective optimization is useful in assessing the suitability of a microorganism as a host strain for the production of a homo-organic acid. The systems metabolic engineering-based approach reported here should be
Original languageEnglish
JournalMetabolic Engineering
Volume28
Pages (from-to)63-73
Number of pages11
ISSN1096-7176
DOIs
Publication statusPublished - 2015

Keywords

  • Byproducts
  • Homo-organic acid
  • Multi-objective optimization
  • Systems metabolic engineering

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