Design of cAMP-CRP-activated promoters in Escherichia coli.

P. Valentin-Hansen; B. Holst; L. Søgaard-Andersen; Jan Martinussen; J. Nesvera; S. R. Douthwaite

doi:10.1111/j.1365-2958.1991.tb02126.x

Design of cAMP-CRP-activated promoters in Escherichia coli.

P. Valentin-Hansen, B. Holst, L. Søgaard-Andersen, Jan Martinussen, J. Nesvera, S. R. Douthwaite

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We have studied the deoP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3',5' monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the -10 hexamer is the crucial factor in determining whether the promoter is activated by cAMP-CRP. Based on these observations, we propose that cAMP-CRP-activated promoters can be created by correctly aligning a CRP target and a -10 hexamer. This idea has been successfully tested by converting both a CRP-independent promoter and a sequence resembling the consensus -10 hexamer to strongly cAMP-CRP-activated promoters.

Original language	English
Journal	Molecular Microbiology
Volume	5
Issue number	2
Pages (from-to)	433-437
Number of pages	5
ISSN	0950-382X
DOIs	https://doi.org/10.1111/j.1365-2958.1991.tb02126.x
Publication status	Published - 1991
Externally published	Yes

Keywords

Base Sequence
Cyclic AMP
DNA, Bacterial
Escherichia coli
Gene Expression Regulation, Bacterial
Molecular Sequence Data
Mutagenesis
Promoter Regions, Genetic
Receptors, Cyclic AMP
E0399OZS9N Cyclic AMP

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title = "Design of cAMP-CRP-activated promoters in Escherichia coli.",

abstract = "We have studied the deoP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3',5' monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the -10 hexamer is the crucial factor in determining whether the promoter is activated by cAMP-CRP. Based on these observations, we propose that cAMP-CRP-activated promoters can be created by correctly aligning a CRP target and a -10 hexamer. This idea has been successfully tested by converting both a CRP-independent promoter and a sequence resembling the consensus -10 hexamer to strongly cAMP-CRP-activated promoters.",

keywords = "Base Sequence, Cyclic AMP, DNA, Bacterial, Escherichia coli, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Mutagenesis, Promoter Regions, Genetic, Receptors, Cyclic AMP, E0399OZS9N Cyclic AMP",

author = "P. Valentin-Hansen and B. Holst and L. S{\o}gaard-Andersen and Jan Martinussen and J. Nesvera and Douthwaite, {S. R.}",

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doi = "10.1111/j.1365-2958.1991.tb02126.x",

language = "English",

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T1 - Design of cAMP-CRP-activated promoters in Escherichia coli.

AU - Valentin-Hansen, P.

AU - Holst, B.

AU - Søgaard-Andersen, L.

AU - Martinussen, Jan

AU - Nesvera, J.

AU - Douthwaite, S. R.

PY - 1991

Y1 - 1991

N2 - We have studied the deoP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3',5' monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the -10 hexamer is the crucial factor in determining whether the promoter is activated by cAMP-CRP. Based on these observations, we propose that cAMP-CRP-activated promoters can be created by correctly aligning a CRP target and a -10 hexamer. This idea has been successfully tested by converting both a CRP-independent promoter and a sequence resembling the consensus -10 hexamer to strongly cAMP-CRP-activated promoters.

AB - We have studied the deoP2 promoter of Escherichia coli to define features that are required for optimal activation by the complex of adenosine 3',5' monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis of deoP2 shows that the distance between the CRP site and the -10 hexamer is the crucial factor in determining whether the promoter is activated by cAMP-CRP. Based on these observations, we propose that cAMP-CRP-activated promoters can be created by correctly aligning a CRP target and a -10 hexamer. This idea has been successfully tested by converting both a CRP-independent promoter and a sequence resembling the consensus -10 hexamer to strongly cAMP-CRP-activated promoters.

KW - Base Sequence

KW - Cyclic AMP

KW - DNA, Bacterial

KW - Escherichia coli

KW - Gene Expression Regulation, Bacterial

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Promoter Regions, Genetic

KW - Receptors, Cyclic AMP

KW - E0399OZS9N Cyclic AMP

U2 - 10.1111/j.1365-2958.1991.tb02126.x

DO - 10.1111/j.1365-2958.1991.tb02126.x

M3 - Journal article

SN - 0950-382X

VL - 5

SP - 433

EP - 437

JO - Molecular Microbiology

JF - Molecular Microbiology

IS - 2

ER -

Design of cAMP-CRP-activated promoters in Escherichia coli.

Abstract

Keywords

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