Abstract. Microarrays are a common method for evaluating genomic content of bacterial species and comparing unsequenced bacterial genomes. This technology allows for quick scans of characteristic genes and chromosomal regions, and to search for indications of horizontal transfer. A high-density microarray chip has been designed, using 116 Enterobacteriaceae genome sequences, taking into account the enteric pan-genome. Probes for the microarray were checked in silico and performance of the chip, based on experimental strains from four different genera, demonstrate a relatively high ability to distinguish those strains on genus, species, and pathotype/serovar levels. Additionally, the microarray performed well when investigating which genes were found in a given strain of interest. The Enterobacteriaceae pan-genome microarray, based on 116 genomes, provides a valuable tool for determination of the genetic makeup of unknown strains within this bacterial family and can introduce insights into phylogenetic relationships.
|Title of host publication||CSBio 2010|
|Publication status||Published - 2010|
|Event||1st International Conference on Computational Systems-Biology and Bioinformatics - Bangkok, Thailand|
Duration: 3 Nov 2010 → 5 Nov 2010
Conference number: 1
|Conference||1st International Conference on Computational Systems-Biology and Bioinformatics|
|Period||03/11/2010 → 05/11/2010|