Design and Validation of Conditional Ligands
for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01,
and HLA-B*44:05

Thomas Mørch Frøsig; Jiawei Yap; Tina Seremet; Rikke Birgitte Lyngaa; Inge Marie Svane; Per Thor Straten; Mirjam H. M. Heemskerk; Gijsbert M. Grotenbreg; Sine Reker Hadrup

doi:10.1002/cyto.a.22689

Design and Validation of Conditional Ligands for HLA-B08:01, HLA-B15:01, HLA-B35:01, and HLA-B44:05

Thomas Mørch Frøsig, Jiawei Yap, Tina Seremet, Rikke Birgitte Lyngaa, Inge Marie Svane, Per Thor Straten, Mirjam H. M. Heemskerk, Gijsbert M. Grotenbreg, Sine Reker Hadrup

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We designed conditional ligands restricted to HLA-B*08:01, 2B*35:01, and 2B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

Original language	English
Journal	Cytometry. Part A
Volume	87
Issue number	10
Pages (from-to)	967-975
Number of pages	9
ISSN	1552-4922
DOIs	https://doi.org/10.1002/cyto.a.22689
Publication status	Published - 2015

Keywords

CD8 T cells
MHC multimer
HLA-B molecules
Flow cytometry
Conditional ligands

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@article{771e78826d3644178127f12614823020,

title = "Design and Validation of Conditional Ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05",

abstract = "We designed conditional ligands restricted to HLA-B*08:01, 2B*35:01, and 2B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.",

keywords = "CD8 T cells, MHC multimer, HLA-B molecules, Flow cytometry, Conditional ligands",

author = "Fr{\o}sig, {Thomas M{\o}rch} and Jiawei Yap and Tina Seremet and Lyngaa, {Rikke Birgitte} and Svane, {Inge Marie} and Straten, {Per Thor} and Heemskerk, {Mirjam H. M.} and {M. Grotenbreg}, Gijsbert and Hadrup, {Sine Reker}",

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T1 - Design and Validation of Conditional Ligands for HLA-B*08:01, HLA-B*15:01, HLA-B*35:01, and HLA-B*44:05

AU - Frøsig, Thomas Mørch

AU - Yap, Jiawei

AU - Seremet, Tina

AU - Lyngaa, Rikke Birgitte

AU - Svane, Inge Marie

AU - Straten, Per Thor

AU - Heemskerk, Mirjam H. M.

AU - M. Grotenbreg, Gijsbert

AU - Hadrup, Sine Reker

PY - 2015

Y1 - 2015

N2 - We designed conditional ligands restricted to HLA-B*08:01, 2B*35:01, and 2B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

AB - We designed conditional ligands restricted to HLA-B*08:01, 2B*35:01, and 2B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.

KW - CD8 T cells

KW - MHC multimer

KW - HLA-B molecules

KW - Flow cytometry

KW - Conditional ligands

U2 - 10.1002/cyto.a.22689

DO - 10.1002/cyto.a.22689

M3 - Journal article

SN - 1552-4922

VL - 87

SP - 967

EP - 975

JO - Cytometry. Part A

JF - Cytometry. Part A

IS - 10

ER -