Design and characterization of self-assembled fish sarcoplasmic protein-alginate nanocomplexes

Karen Boutrup Stephansen, Maria Ahlm Mattebjerg, Jasper Wattjes, Ana Milisavljevic, Flemming Jessen, Klaus Qvortrup, Francisco M. Goycoolea, Ioannis S. Chronakis

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Macrostructures based on natural polymers are subject to large attention, as the application range is wide within the food and pharmaceutical industries. In this study we present nanocomplexes (NCXs) made from electrostatic self-assembly between negatively charged alginate and positively charged fish sarcoplasmic proteins (FSP), prepared by bulk mixing. A concentration screening revealed that there was a range of alginate and FSP concentrations where stable NCXs with similar properties were formed, rather than two exact concentrations. The size of the NCXs was 293 +/- 3 nm, and the zeta potential was -42 +/- 0.3 mV. The NCXs were stable in water, gastric buffer, intestinal buffer and HEPES buffered glycose, and at all pH values from 2 to 9 except pH 3, where they aggregated. When proteolytic enzymes were present in the buffer, the NCXs were degraded. Only at high concentrations the NCXs caused a decreased viability in HeLa and U2OS cell lines. The simple processing procedure and the high stability of the NCXs, makes them excellent candidates for use in the food and pharmaceutical industry. (C) 2015 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Volume76
Pages (from-to)146-152
Number of pages7
ISSN0141-8130
DOIs
Publication statusPublished - 2015

Keywords

  • Alginate
  • Proteins
  • Particle
  • Nanocomplexd
  • Drug delivery

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