Desaturation of skeletal muscle structural and depot lipids in obese individuals during a very-low-calorie diet intervention

S.B. Haugaard, A. Vaag, Carl-Erik Høy, S. Madsbad

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Objective: This study investigated whether a very-low-calorie dietary intervention (VLCD) may influence composition of skeletal muscle cell membrane phospholipid and composition and concentration of intramyocellular triglyceride (IMTG) in obese subjects. The working hypothesis proposed that a VLCD would decrease saturated fatty acids (FAs) and increase long-chain polyunsaturated FAs (LCPUFAs) in muscular structural lipids, as such changes have been associated with improved insulin sensitivity. Research Methods and Procedures: Skeletal muscle biopsies (vastus lateralis) were obtained from 13 obese subjects (nine women) before and after 8 weeks on VLCD ( similar to 600 to 800 kcal/d). FA composition in muscle cell membrane phospholipid and concentration and FA composition of IMTG were determined by gas-liquid chromatography. Results: Baseline BMI was 36.0 +/- 3.4 kg/m(2). Weight loss was 9.3 +/- 1.1 kg (8.8 +/- 1. 1%; p <0.000 1); loss of adipose tissue was 5.9 +/- 0.9 kg (p <0.0001). Insulin resistance (by homeostasis model assessment) decreased (-44 +/- 7%; p <0.001). Muscle cell membrane phospholipid saturated FAs decreased (-3.2 +/- 1.3%; p <0.05), whereas monounsaturated FAs (4.3 +/- 1.7%; p. <0.05), LCPUFAs (11 +/- 6%; p <0.05), and the ratio of LCPUFAs to saturated FAs (12 +/- 5%; p <0.05) increased. IMTG decreased, but not significantly (-5%). IMTG-saturated FAs decreased (-3.3 +/- 1.5%; p <0.05), whereas LCPUFAn-3 (29 +/- 9%; p <0.01), LCPUFAn-6 (33 +/- 9%; p <0.01), and the ratio of LCPUFAs to saturated FAs (34 +/- 8%; 1) p <0.001) increased. Plasma total cholesterol (-15 +/- 6%; p <0.05), low-density lipoprotein-cholesterol (-16 +/- 5%; p <0.01), high-density lipoprotein-cholesterol (-8 +/- 2%; p <0.01), and plasma triglyceride (-19 +/- 12%; p = 0.10) all decreased during the VLCD. Discussion: Desaturation of both muscle cell membrane phospholipid and IMTG was significant but modest during a VLCD in obese subjects. Further research must delineate whether such changes in skeletal muscle structural and depot lipid composition themselves are enough to promote the observed improvements in insulin action.
    Original languageEnglish
    JournalObesity
    Volume15
    Issue number1
    Pages (from-to)117-125
    ISSN1930-7381
    DOIs
    Publication statusPublished - 2007

    Keywords

    • membrane phospholipids
    • weight loss
    • insulin sensitivity
    • dietary intervention
    • intramyocellular triglyceride

    Cite this

    Haugaard, S.B. ; Vaag, A. ; Høy, Carl-Erik ; Madsbad, S. / Desaturation of skeletal muscle structural and depot lipids in obese individuals during a very-low-calorie diet intervention. In: Obesity. 2007 ; Vol. 15, No. 1. pp. 117-125.
    @article{0a9a955c729f4039bfec0c0a985e8392,
    title = "Desaturation of skeletal muscle structural and depot lipids in obese individuals during a very-low-calorie diet intervention",
    abstract = "Objective: This study investigated whether a very-low-calorie dietary intervention (VLCD) may influence composition of skeletal muscle cell membrane phospholipid and composition and concentration of intramyocellular triglyceride (IMTG) in obese subjects. The working hypothesis proposed that a VLCD would decrease saturated fatty acids (FAs) and increase long-chain polyunsaturated FAs (LCPUFAs) in muscular structural lipids, as such changes have been associated with improved insulin sensitivity. Research Methods and Procedures: Skeletal muscle biopsies (vastus lateralis) were obtained from 13 obese subjects (nine women) before and after 8 weeks on VLCD ( similar to 600 to 800 kcal/d). FA composition in muscle cell membrane phospholipid and concentration and FA composition of IMTG were determined by gas-liquid chromatography. Results: Baseline BMI was 36.0 +/- 3.4 kg/m(2). Weight loss was 9.3 +/- 1.1 kg (8.8 +/- 1. 1{\%}; p <0.000 1); loss of adipose tissue was 5.9 +/- 0.9 kg (p <0.0001). Insulin resistance (by homeostasis model assessment) decreased (-44 +/- 7{\%}; p <0.001). Muscle cell membrane phospholipid saturated FAs decreased (-3.2 +/- 1.3{\%}; p <0.05), whereas monounsaturated FAs (4.3 +/- 1.7{\%}; p. <0.05), LCPUFAs (11 +/- 6{\%}; p <0.05), and the ratio of LCPUFAs to saturated FAs (12 +/- 5{\%}; p <0.05) increased. IMTG decreased, but not significantly (-5{\%}). IMTG-saturated FAs decreased (-3.3 +/- 1.5{\%}; p <0.05), whereas LCPUFAn-3 (29 +/- 9{\%}; p <0.01), LCPUFAn-6 (33 +/- 9{\%}; p <0.01), and the ratio of LCPUFAs to saturated FAs (34 +/- 8{\%}; 1) p <0.001) increased. Plasma total cholesterol (-15 +/- 6{\%}; p <0.05), low-density lipoprotein-cholesterol (-16 +/- 5{\%}; p <0.01), high-density lipoprotein-cholesterol (-8 +/- 2{\%}; p <0.01), and plasma triglyceride (-19 +/- 12{\%}; p = 0.10) all decreased during the VLCD. Discussion: Desaturation of both muscle cell membrane phospholipid and IMTG was significant but modest during a VLCD in obese subjects. Further research must delineate whether such changes in skeletal muscle structural and depot lipid composition themselves are enough to promote the observed improvements in insulin action.",
    keywords = "membrane phospholipids, weight loss, insulin sensitivity, dietary intervention, intramyocellular triglyceride",
    author = "S.B. Haugaard and A. Vaag and Carl-Erik H{\o}y and S. Madsbad",
    year = "2007",
    doi = "10.1038/oby.2007.508",
    language = "English",
    volume = "15",
    pages = "117--125",
    journal = "Obesity",
    issn = "1930-7381",
    publisher = "Wiley-Blackwell",
    number = "1",

    }

    Desaturation of skeletal muscle structural and depot lipids in obese individuals during a very-low-calorie diet intervention. / Haugaard, S.B.; Vaag, A.; Høy, Carl-Erik; Madsbad, S.

    In: Obesity, Vol. 15, No. 1, 2007, p. 117-125.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Desaturation of skeletal muscle structural and depot lipids in obese individuals during a very-low-calorie diet intervention

    AU - Haugaard, S.B.

    AU - Vaag, A.

    AU - Høy, Carl-Erik

    AU - Madsbad, S.

    PY - 2007

    Y1 - 2007

    N2 - Objective: This study investigated whether a very-low-calorie dietary intervention (VLCD) may influence composition of skeletal muscle cell membrane phospholipid and composition and concentration of intramyocellular triglyceride (IMTG) in obese subjects. The working hypothesis proposed that a VLCD would decrease saturated fatty acids (FAs) and increase long-chain polyunsaturated FAs (LCPUFAs) in muscular structural lipids, as such changes have been associated with improved insulin sensitivity. Research Methods and Procedures: Skeletal muscle biopsies (vastus lateralis) were obtained from 13 obese subjects (nine women) before and after 8 weeks on VLCD ( similar to 600 to 800 kcal/d). FA composition in muscle cell membrane phospholipid and concentration and FA composition of IMTG were determined by gas-liquid chromatography. Results: Baseline BMI was 36.0 +/- 3.4 kg/m(2). Weight loss was 9.3 +/- 1.1 kg (8.8 +/- 1. 1%; p <0.000 1); loss of adipose tissue was 5.9 +/- 0.9 kg (p <0.0001). Insulin resistance (by homeostasis model assessment) decreased (-44 +/- 7%; p <0.001). Muscle cell membrane phospholipid saturated FAs decreased (-3.2 +/- 1.3%; p <0.05), whereas monounsaturated FAs (4.3 +/- 1.7%; p. <0.05), LCPUFAs (11 +/- 6%; p <0.05), and the ratio of LCPUFAs to saturated FAs (12 +/- 5%; p <0.05) increased. IMTG decreased, but not significantly (-5%). IMTG-saturated FAs decreased (-3.3 +/- 1.5%; p <0.05), whereas LCPUFAn-3 (29 +/- 9%; p <0.01), LCPUFAn-6 (33 +/- 9%; p <0.01), and the ratio of LCPUFAs to saturated FAs (34 +/- 8%; 1) p <0.001) increased. Plasma total cholesterol (-15 +/- 6%; p <0.05), low-density lipoprotein-cholesterol (-16 +/- 5%; p <0.01), high-density lipoprotein-cholesterol (-8 +/- 2%; p <0.01), and plasma triglyceride (-19 +/- 12%; p = 0.10) all decreased during the VLCD. Discussion: Desaturation of both muscle cell membrane phospholipid and IMTG was significant but modest during a VLCD in obese subjects. Further research must delineate whether such changes in skeletal muscle structural and depot lipid composition themselves are enough to promote the observed improvements in insulin action.

    AB - Objective: This study investigated whether a very-low-calorie dietary intervention (VLCD) may influence composition of skeletal muscle cell membrane phospholipid and composition and concentration of intramyocellular triglyceride (IMTG) in obese subjects. The working hypothesis proposed that a VLCD would decrease saturated fatty acids (FAs) and increase long-chain polyunsaturated FAs (LCPUFAs) in muscular structural lipids, as such changes have been associated with improved insulin sensitivity. Research Methods and Procedures: Skeletal muscle biopsies (vastus lateralis) were obtained from 13 obese subjects (nine women) before and after 8 weeks on VLCD ( similar to 600 to 800 kcal/d). FA composition in muscle cell membrane phospholipid and concentration and FA composition of IMTG were determined by gas-liquid chromatography. Results: Baseline BMI was 36.0 +/- 3.4 kg/m(2). Weight loss was 9.3 +/- 1.1 kg (8.8 +/- 1. 1%; p <0.000 1); loss of adipose tissue was 5.9 +/- 0.9 kg (p <0.0001). Insulin resistance (by homeostasis model assessment) decreased (-44 +/- 7%; p <0.001). Muscle cell membrane phospholipid saturated FAs decreased (-3.2 +/- 1.3%; p <0.05), whereas monounsaturated FAs (4.3 +/- 1.7%; p. <0.05), LCPUFAs (11 +/- 6%; p <0.05), and the ratio of LCPUFAs to saturated FAs (12 +/- 5%; p <0.05) increased. IMTG decreased, but not significantly (-5%). IMTG-saturated FAs decreased (-3.3 +/- 1.5%; p <0.05), whereas LCPUFAn-3 (29 +/- 9%; p <0.01), LCPUFAn-6 (33 +/- 9%; p <0.01), and the ratio of LCPUFAs to saturated FAs (34 +/- 8%; 1) p <0.001) increased. Plasma total cholesterol (-15 +/- 6%; p <0.05), low-density lipoprotein-cholesterol (-16 +/- 5%; p <0.01), high-density lipoprotein-cholesterol (-8 +/- 2%; p <0.01), and plasma triglyceride (-19 +/- 12%; p = 0.10) all decreased during the VLCD. Discussion: Desaturation of both muscle cell membrane phospholipid and IMTG was significant but modest during a VLCD in obese subjects. Further research must delineate whether such changes in skeletal muscle structural and depot lipid composition themselves are enough to promote the observed improvements in insulin action.

    KW - membrane phospholipids

    KW - weight loss

    KW - insulin sensitivity

    KW - dietary intervention

    KW - intramyocellular triglyceride

    U2 - 10.1038/oby.2007.508

    DO - 10.1038/oby.2007.508

    M3 - Journal article

    C2 - 17228039

    VL - 15

    SP - 117

    EP - 125

    JO - Obesity

    JF - Obesity

    SN - 1930-7381

    IS - 1

    ER -