TY - JOUR
T1 - Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001.
AU - Devi, Sagarika
AU - Kiesewalter, Heiko T.
AU - Kovács, Renátó
AU - Frisvad, Jens Christian
AU - Weber, Tilmann
AU - Larsen, Thomas Ostenfeld
AU - Kovács, Ákos T.
AU - Ding, Ling
PY - 2019
Y1 - 2019
N2 - For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
AB - For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
KW - Bacillus velezensis
KW - Candida albicans
KW - Secondary metabolites
KW - Biocontrol
KW - Biofilm
U2 - 10.1016/j.synbio.2019.08.002
DO - 10.1016/j.synbio.2019.08.002
M3 - Journal article
C2 - 31508511
SN - 2405-805x
VL - 4
SP - 142
EP - 149
JO - Synthetic and Systems Biotechnology
JF - Synthetic and Systems Biotechnology
IS - 3
ER -