Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001.

Sagarika Devi, Heiko T. Kiesewalter, Renátó Kovács, Jens Christian Frisvad, Tilmann Weber, Thomas Ostenfeld Larsen, Ákos T. Kovács, Ling Ding*

*Corresponding author for this work

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Abstract

For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
Original languageEnglish
JournalSynthetic and Systems Biotechnology
Volume4
Issue number3
Pages (from-to)142-149
Number of pages8
ISSN2405-805x
DOIs
Publication statusPublished - 2019

Keywords

  • Bacillus velezensis
  • Candida albicans
  • Secondary metabolites
  • Biocontrol
  • Biofilm

Cite this

@article{6dcd37d45d2d424d91c80e19d6e4aff0,
title = "Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001.",
abstract = "For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.",
keywords = "Bacillus velezensis, Candida albicans, Secondary metabolites, Biocontrol, Biofilm",
author = "Sagarika Devi and Kiesewalter, {Heiko T.} and Ren{\'a}t{\'o} Kov{\'a}cs and Frisvad, {Jens Christian} and Tilmann Weber and Larsen, {Thomas Ostenfeld} and Kov{\'a}cs, {{\'A}kos T.} and Ling Ding",
year = "2019",
doi = "10.1016/j.synbio.2019.08.002",
language = "English",
volume = "4",
pages = "142--149",
journal = "Synthetic and Systems Biotechnology",
issn = "2405-805x",
publisher = "KeAi Communications Co",
number = "3",

}

Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001. / Devi, Sagarika; Kiesewalter, Heiko T.; Kovács, Renátó; Frisvad, Jens Christian; Weber, Tilmann; Larsen, Thomas Ostenfeld; Kovács, Ákos T.; Ding, Ling.

In: Synthetic and Systems Biotechnology, Vol. 4, No. 3, 2019, p. 142-149.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001.

AU - Devi, Sagarika

AU - Kiesewalter, Heiko T.

AU - Kovács, Renátó

AU - Frisvad, Jens Christian

AU - Weber, Tilmann

AU - Larsen, Thomas Ostenfeld

AU - Kovács, Ákos T.

AU - Ding, Ling

PY - 2019

Y1 - 2019

N2 - For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.

AB - For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.

KW - Bacillus velezensis

KW - Candida albicans

KW - Secondary metabolites

KW - Biocontrol

KW - Biofilm

U2 - 10.1016/j.synbio.2019.08.002

DO - 10.1016/j.synbio.2019.08.002

M3 - Journal article

VL - 4

SP - 142

EP - 149

JO - Synthetic and Systems Biotechnology

JF - Synthetic and Systems Biotechnology

SN - 2405-805x

IS - 3

ER -