deFUME: Dynamic exploration of functional metagenomic sequencing data

Eric van der Helm, Henrik Marcus Geertz-Hansen, Hans Jasper Genee, Sailesh Malla, Morten Otto Alexander Sommer

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Functional metagenomic selections represent a powerful technique that is widely applied for identification of novel genes from complex metagenomic sources. However, whereas hundreds to thousands of clones can be easily generated and sequenced over a few days of experiments, analyzing the data is time consuming and constitutes a major bottleneck for experimental researchers in the field. Here we present the deFUME web server, an easy-to-use web-based interface for processing, annotation and visualization of functional metagenomics sequencing data, tailored to meet the requirements of non-bioinformaticians. The web-server integrates multiple analysis steps into one single workflow: read assembly, open reading frame prediction, and annotation with BLAST, InterPro and GO classifiers. Analysis results are visualized in an online dynamic web-interface. The deFUME webserver provides a fast track from raw sequence to a comprehensive visual data overview that facilitates effortless inspection of gene function, clustering and distribution. The webserver is available at cbs.dtu.dk/services/deFUME/and the source code is distributed at github.com/EvdH0/deFUME.
Original languageEnglish
JournalBMC Research Notes
Volume8
Issue number328
Pages (from-to)328
Number of pages1
ISSN1756-0500
DOIs
Publication statusPublished - 2015

Bibliographical note

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons. org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Keywords

  • Functional metagenomics
  • Web services
  • Visualization
  • Sequence analysis
  • Gene ontology

Cite this

van der Helm, Eric ; Geertz-Hansen, Henrik Marcus ; Genee, Hans Jasper ; Malla, Sailesh ; Sommer, Morten Otto Alexander. / deFUME: Dynamic exploration of functional metagenomic sequencing data. In: BMC Research Notes. 2015 ; Vol. 8, No. 328. pp. 328.
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deFUME: Dynamic exploration of functional metagenomic sequencing data. / van der Helm, Eric; Geertz-Hansen, Henrik Marcus; Genee, Hans Jasper; Malla, Sailesh; Sommer, Morten Otto Alexander.

In: BMC Research Notes, Vol. 8, No. 328, 2015, p. 328.

Research output: Contribution to journalJournal articleResearchpeer-review

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