Abstract
An optical two-beam trap composed from two counter propagating laser beams is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named the optical stretcher by Jochen Guck, Josep Kaas and co-workers almost 20 years ago. In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may ow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world. The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we discuss advantages and disadvantages of choice of material and methodology for chip assembly and chip production. For high throughput investigations of stretching deformation of single cells, optical stretching is, however, out-performed by hydrodynamic deformability assays. As we will discuss, injection molded polymer chips may with advantage be applied both for optical stretching and for hydrodynamic deformability experiments.
| Original language | English |
|---|---|
| Title of host publication | Complex Light and Optical Forces XIII |
| Editors | Enrique J. Galvez, David L. Andrews, Jesper Gluckstad |
| Number of pages | 9 |
| Publisher | SPIE |
| Publication date | 2019 |
| Article number | 1093516 |
| ISBN (Electronic) | 9781510625129 |
| DOIs | |
| Publication status | Published - 2019 |
| Event | Complex Light and Optical Forces XIII - The Moscone Center, San Francisco, United States Duration: 5 Feb 2019 → 7 Feb 2019 Conference number: SPIE Conference 10935 |
Conference
| Conference | Complex Light and Optical Forces XIII |
|---|---|
| Number | SPIE Conference 10935 |
| Location | The Moscone Center |
| Country | United States |
| City | San Francisco |
| Period | 05/02/2019 → 07/02/2019 |
| Series | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 10935 |
| ISSN | 0277-786X |
Keywords
- Optical and hydrodynamic stretching
- Polymer micro uidic chips
- Red blood cells
- Single cell biophysics
Cite this
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Deformation of single cells - Optical two-beam traps and more. / Nielsen, Kirstine Sandager; Rungling, Tony B.; Dziegiel, Morten Hanefeld; Marie, Rodolphe; Berg-Sørensen, Kirstine.
Complex Light and Optical Forces XIII. ed. / Enrique J. Galvez; David L. Andrews; Jesper Gluckstad. SPIE, 2019. 1093516 (Proceedings of SPIE - The International Society for Optical Engineering, Vol. 10935).Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
TY - GEN
T1 - Deformation of single cells - Optical two-beam traps and more
AU - Nielsen, Kirstine Sandager
AU - Rungling, Tony B.
AU - Dziegiel, Morten Hanefeld
AU - Marie, Rodolphe
AU - Berg-Sørensen, Kirstine
PY - 2019
Y1 - 2019
N2 - An optical two-beam trap composed from two counter propagating laser beams is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named the optical stretcher by Jochen Guck, Josep Kaas and co-workers almost 20 years ago. In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may ow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world. The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we discuss advantages and disadvantages of choice of material and methodology for chip assembly and chip production. For high throughput investigations of stretching deformation of single cells, optical stretching is, however, out-performed by hydrodynamic deformability assays. As we will discuss, injection molded polymer chips may with advantage be applied both for optical stretching and for hydrodynamic deformability experiments.
AB - An optical two-beam trap composed from two counter propagating laser beams is an interesting setup due to the ability of the system to trap, hold, and stretch soft biological objects like vesicles or single cells. Because of this functionality, the system was also named the optical stretcher by Jochen Guck, Josep Kaas and co-workers almost 20 years ago. In a favorable setup, the two opposing laser beams meet with equal intensities in the middle of a fluidic channel in which cells may ow past, be trapped, stretched, and allowed to move on, giving the promise of a high throughput device. Yet, single beam optical traps, aka optical tweezers, by far outnumber the existing optical stretchers in research labs throughout the world. The ability to easily construct an optical stretcher setup in a low-cost material would possibly imply more frequent use of the optical stretching technique. Here, we discuss advantages and disadvantages of choice of material and methodology for chip assembly and chip production. For high throughput investigations of stretching deformation of single cells, optical stretching is, however, out-performed by hydrodynamic deformability assays. As we will discuss, injection molded polymer chips may with advantage be applied both for optical stretching and for hydrodynamic deformability experiments.
KW - Optical and hydrodynamic stretching
KW - Polymer micro uidic chips
KW - Red blood cells
KW - Single cell biophysics
U2 - 10.1117/12.2513407
DO - 10.1117/12.2513407
M3 - Article in proceedings
BT - Complex Light and Optical Forces XIII
A2 - Galvez, Enrique J.
A2 - Andrews, David L.
A2 - Gluckstad, Jesper
PB - SPIE
ER -