Definition of core bacterial taxa in different root compartments of dactylis glomerata, grown in soil under different levels of land use intensity

Jennifer Estendorfer, Barbara Stempfhuber*, Gisle Alberg Vestergaard, Stefanie Schulz, Matthias C. Rillig, Jasmin Joshi, Peter Schröder, Michael Schloter

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Plant-associated bacterial assemblages are critical for plant fitness. Thus, identifying a consistent plant-associated core microbiome is important for predicting community responses to environmental changes. Our target was to identify the core bacterial microbiome of orchard grass Dactylis glomerata L. and to assess the part that is most sensitive to land management. Dactylis glomerata L. samples were collected from grassland sites with contrasting land use intensities but comparable soil properties at three different timepoints. To assess the plant-associated bacterial community structure in the compartments rhizosphere, bulk soil and endosphere, a molecular barcoding approach based on high throughput 16S rRNA amplicon sequencing was used. A distinct composition of plant-associated core bacterial communities independent of land use intensity was identified. Pseudomonas, Rhizobium and Bradyrhizobium were ubiquitously found in the root bacterial core microbiome. In the rhizosphere, the majority of assigned genera were Rhodoplanes, Methylibium, Kaistobacter and Bradyrhizobium. Due to the frequent occurrence of plant-promoting abilities in the genera found in the plant-associated core bacterial communities, our study helps to identify “healthy” plant-associated bacterial core communities. The variable part of the plant-associated microbiome, represented by the fluctuation of taxa at the different sampling timepoints, was increased under low land use intensity. This higher compositional variation in samples from plots with low land use intensity indicates a more selective recruitment of bacteria with traits required at different timepoints of plant development compared to samples from plots with high land use intensity.
Original languageEnglish
Article number392
JournalDiversity
Volume12
Issue number10
Number of pages18
ISSN1424-2818
DOIs
Publication statusPublished - 2020

Keywords

  • Land use intensity
  • Plant-associated microbiome
  • Endophytes
  • Rhizosphere
  • Biodiversity
  • Bacteria
  • Core microbiome
  • Pseudomonas

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