Defining the HLA class I-associated viral antigen repertoire from HIV-1-infected human cells

Research output: Contribution to journalJournal article – Annual report year: 2015Researchpeer-review

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DOI

  • Author: Ternette, Nicola

    University of Oxford, United Kingdom

  • Author: Yang, Hongbing

    NIHR Oxford Biomedical Research Centre, United Kingdom

  • Author: Partridge, Thomas

    University of Oxford, United Kingdom

  • Author: Llano, Anuska

    Autonomous University of Barcelona, Spain

  • Author: Cedeño, Samandhy

    Autonomous University of Barcelona, Spain

  • Author: Fischer, Roman

    University of Oxford, United Kingdom

  • Author: Charles, Philip D.

    University of Oxford, United Kingdom

  • Author: Dudek, Nadine L.

    Monash University, Australia

  • Author: Mothe, Beatriz

    Autonomous University of Barcelona, Spain

  • Author: Crespo, Manuel

    University Hospital Vall d’Hebron, Spain

  • Author: Fischer, William M.

    Los Alamos National Laboratory, United States

  • Author: Korber, Bette T. M.

    Los Alamos National Laboratory, United States

  • Author: Nielsen, Morten

    Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark

  • Author: Borrow, Persephone

    University of Oxford, United Kingdom

  • Author: Purcell, Anthony W.

    Monash University, Australia

  • Author: Brander, Christian

    Autonomous University of Barcelona, Spain

  • Author: Dorrell, Lucy

    University of Oxford, United Kingdom

  • Author: Kessler, Benedikt M.

    University of Oxford, United Kingdom

  • Author: Hanke, Tomáš

    University of Oxford, United Kingdom

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Recognition and eradication of infected cells by cytotoxic T lymphocytes is a key defense mechanism against intracellular pathogens. High-throughput definition of HLA class I-associated immunopeptidomes by mass spectrometry is an increasingly important analytical tool to advance our understanding of the induction of T-cell responses against pathogens such as HIV-1. We utilized a liquid chromatography tandem mass spectrometry workflow including de novo-assisted database searching to define the HLA class I-associated immunopeptidome of HIV-1-infected human cells. We here report for the first time the identification of 75 HIV-1-derived peptides bound to HLA class I complexes that were purified directly from HIV-1-infected human primary CD4+ T cells and the C8166 human T-cell line. Importantly, one-third of eluted HIV-1 peptides had not been previously known to be presented by HLA class I. Over 82% of the identified sequences originated from viral protein regions for which T-cell responses have previously been reported but for which the precise HLA class I-binding sequences have not yet been defined. These results validate and expand the current knowledge of virus-specific antigenic peptide presentation during HIV-1 infection and provide novel targets for T-cell vaccine development.
Original languageEnglish
JournalEuropean Journal of Immunology
Volume46
Issue number1
Pages (from-to)60-69
Number of pages10
ISSN0014-2980
DOIs
Publication statusPublished - 2016

Bibliographical note

© 2015 The Authors. European Journal of Immunology published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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    Research areas

  • Cytotoxic T cells, Human immunodeficiency virus type I, Human leukocyte antigen, Immunopeptidome, Mass spectrometry

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