Decolorization and detoxification of sulfonated azo dye methyl orange by Kocuria rosea MTCC 1532

G.K. Parshetti, A.A. Telke, Dayanand Kalyani, S.P. Govindwar

Research output: Contribution to journalJournal articleResearchpeer-review


Kocuria rosea (MTCC 1532) showed 100% decolorization of methyl orange (50mgl−1) under static condition.
The optimum pH and temperature for dye decolorization was 6.8 and 30 ◦C, respectively. The K. rosea
(MTCC 1532) showed maximum decolorization of methyl orange when growth medium containing yeast
extract as compared to other substrates. The culture exhibited significant ability to decolorize repeated
additions of dye, with reduction in time up to 12 h at eighth dye aliquot addition. Significant induction
of reductases (NADH-DCIP reductase and azoreductase) suggests its involvement in decolorization of
methyl orange. The metabolites formed after decolorization of methyl orange, such as 4-amino sulfonic
acid and N,N-dimethyl p-phenyldiamine were characterized using FTIR and MS. Phytotoxicity and microbial
toxicity study showed the methyl orange was toxic and metabolites obtained after its decolorization
was nontoxic for experimental plants (Triticum aestivum and Phaseolus mungo) and bacteria (K. rosea,
Pseudomonas aurugenosa and Azatobacter vinelandii).
Original languageEnglish
JournalJournal of Hazardous Materials
Issue number3-3
Pages (from-to)503-509
Publication statusPublished - 2010
Externally publishedYes


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