Dead-box RNA helicases in Bacillus subtilis have multiple functions and Act independently from each other

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T. Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P. Kuipers, Jörg Stülke*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

DEAD-box RNA helicases play important roles in remodeling RNA molecules and in facilitating a variety of RNA-protein interactions that are key to many essential cellular processes. In spite of the importance of RNA, our knowledge about RNA helicases is limited. In this study, we investigated the role of the four DEAD-box RNA helicases in the Gram-positive model organism Bacillus subtilis. A strain deleted of all RNA helicases is able to grow at 37°C but not at lower temperatures. The deletion of cshA, cshB, or yfmL in particular leads to cold-sensitive phenotypes. Moreover, these mutant strains exhibit unique defects in ribosome biogenesis, suggesting distinct functions for the individual enzymes in this process. Based on protein accumulation, severity of the cold-sensitive phenotype, and the interaction with components of the RNA degradosome, CshA is the major RNA helicase of B. subtilis. To unravel the functions of CshA in addition to ribosome biogenesis, we conducted microarray analysis and identified the ysbAB and frlBONMD mRNAs as targets that are strongly affected by the deletion of the cshA gene. Our findings suggest that the different helicases make distinct contributions to the physiology of B. subtilis. Ribosome biogenesis and RNA degradation are two of their major tasks in B. subtilis.

Original languageEnglish
JournalJournal of Bacteriology
Volume195
Issue number3
Pages (from-to)534-544
ISSN0021-9193
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this

Lehnik-Habrink, M., Rempeters, L., Kovács, Á. T., Wrede, C., Baierlein, C., Krebber, H., Kuipers, O. P., & Stülke, J. (2013). Dead-box RNA helicases in Bacillus subtilis have multiple functions and Act independently from each other. Journal of Bacteriology, 195(3), 534-544. https://doi.org/10.1128/JB.01475-12