De novo biofilm community assembly from tap water source communities favors Nitrotoga over Nitrospira under elevated nitrite surface loading

    Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

    357 Downloads (Pure)

    Abstract

    Four main processes are considered to drive microbial community assembly: selection, drift, dispersal and speciation. These processes occur simultaneously, but the extent to which each process contributes to community assembly is unclear in natural communities. We exposed a high-throughput flow-through biofilm system to continuous immigration from a tap water metacommunity while applying different nitrite surface loading rates. After 63 days of operation, we extracted biofilms and analyzed the community composition via Illumina MiSeq targeting the 16S rRNA gene. Previous studies have shown that Nitrospira is the dominant nitrite oxidizing genus in low nitrite environments. Hence, we postulated that by elevating the nitrite surface loading we would select for NOB with lower nitrite affinity than Nitrospira. We observed different dominant NOB species under different loading rates. While in the metacommunity, Nitrotoga and Nitrospira were found at near equal abundances, in the biofilm community, elevated nitrite loading strongly selected for Nitrotoga over Nitrospira. The biofilms were also significantly different in their alpha-diversity (p<0.001) and beta-diversity, and the evenness and richness of the biofilm community decreased significantly (p=0.004) compared to the metacommunity. These observations indicate that the selection towards Nitrotoga and Nitrospira dominated community assembly under different nitrite loadings. Lastly, we compared our observations of community composition with that predicted by neutral community assembly model. The predictions did not match the community structure observed in the biofilms (p=0.31), providing further evidence of the importance of selection during community assembly.
    Original languageEnglish
    Publication date2016
    Number of pages1
    Publication statusPublished - 2016
    Event16th International Symposium on Microbial Ecology - Montreal, Canada
    Duration: 21 Aug 201626 Aug 2016
    Conference number: 16
    http://www.isme-microbes.org/isme16

    Conference

    Conference16th International Symposium on Microbial Ecology
    Number16
    Country/TerritoryCanada
    CityMontreal
    Period21/08/201626/08/2016
    Internet address

    Bibliographical note

    Poster presentation

    Fingerprint

    Dive into the research topics of 'De novo biofilm community assembly from tap water source communities favors Nitrotoga over Nitrospira under elevated nitrite surface loading'. Together they form a unique fingerprint.

    Cite this