Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria

Ilaria Benedetti, Pablo Ivan Nikel, Victor de Lorenzo

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Abstract

Engineering of robust microbial cell factories requires the use of dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. We have edited and formatted the ChnR/PchnB regulatory node of Acinetobacter johnsonii to ease the targeted engineering of ectopic gene expression in Gram-negative bacteria. The proposed compositional standard was thoroughly verified with a monomeric and superfolder green fluorescent protein (msf•GFP) in Escherichia coli. The expression data presented reflect a tightly controlled transcription initiation signal in response to cyclohexanone. Data in this article are related to the research paper "Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes" [1].
Original languageEnglish
JournalData in Brief
Volume6
Pages (from-to)738-744
Number of pages7
ISSN2352-3409
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Escherichia coli
  • Expression system
  • Heterologous gene expression
  • Metabolic engineering
  • Pseudomonas putida
  • Synthetic biology

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